{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:19:09Z","timestamp":1787660349812,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Human papillomavirus (HPV) DNA testing is the recommended primary screening method for cervical cancer prevention. However, implementation in resource\u2010limited and geographically challenging settings is constrained by cost, logistics, and time\u2010sensitive screen\u2010and\u2010treat workflow. Sample pooling may improve testing efficiency, but evidence for its feasibility on point\u2010of\u2010care molecular platforms remains limited. We evaluated the analytical performance and feasibility of sample pooling for high\u2010risk HPV (hrHPV) detection using the STANDARD\u2122 M10 HPV point\u2010of\u2010care assay. Archived self\u2010collected vaginal swab samples from a rural cervical cancer screening programme, Programme SUARA, in Sarawak, Malaysia, were used, including four hrHPV\u2010positive samples spanning a range of cycle threshold (Ct) values and seven hrHPV\u2010negative samples. Pooling strategies of 2\u2010in\u20101, 4\u2010in\u20101, and 8\u2010in\u20101 were assessed using a Dorfman two\u2010stage testing approach. Qualitative agreement, sensitivity, Ct shifts, and concordance with individual testing were analyzed. All hrHPV\u2010positive samples were consistently detected across individual and pooled formats, with no false\u2010negative results observed. Sensitivity remained 100% for all pooling strategies, with perfect agreement between pooled and individual testing (\n                    <jats:italic>\u03ba<\/jats:italic>\n                    \u2009=\u20091.0). Pooling resulted in modest Ct shifts, with mean increases of +1.0, +1.7, and +1.8 cycles for 2\u2010in\u20101, 4\u2010in\u20101, and 8\u2010in\u20101 pools, respectively, without compromising qualitative detection, including samples with low viral loads. Sample pooling for hrHPV DNA testing using the STANDARD\u2122 M10 platform is analytically reliable and operationally feasible. These findings support pooled testing as an evidence\u2010based strategy to improve throughput, reduce resource consumption, and enable same\u2010day screen\u2010and\u2010treat workflows in resource\u2010limited and remote settings.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71101","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:05:41Z","timestamp":1786356341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Sample Pooling Strategies for Point\u2010of\u2010Care HPV DNA Testing Using the STANDARD\u2122 M10 Platform"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3601-7582","authenticated-orcid":false,"given":"Ahmad Asyraf","family":"Senian","sequence":"first","affiliation":[{"name":"Department of Paraclinical Sciences Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Centre for Tropical and Emerging Diseases, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2125-2237","authenticated-orcid":false,"given":"Vaenessa","family":"Noni","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Centre for Tropical and Emerging Diseases, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Therapeutic Research Centre, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail Rembui","family":"Jerip","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Pink and Teal Empowher, Kota Padawan Kuching Sarawak Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2607-1177","authenticated-orcid":false,"given":"Andy Cheong Shin","family":"Bong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dzuriaty","family":"Siri\u2010ee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Pink and Teal Empowher, Kota Padawan Kuching Sarawak Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6312-4551","authenticated-orcid":false,"given":"Cheng Siang","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Centre for Tropical and Emerging Diseases, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Therapeutic Research Centre, Faculty of Medicine and Health Sciences Universiti Malaysia Sarawak Kota Samarahan Sarawak Malaysia"},{"name":"Pink and Teal Empowher, Kota Padawan Kuching Sarawak Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-025-11441-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(19)30488-7"},{"key":"e_1_2_10_4_1","unstructured":"International Agency for Research on Cancer \u201cMalaysia: Human Papillomavirus and Related Cancers Fact Sheet 2023 \u201d2023 www.hpvcentre.net."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-023-17132-2"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-025-10714-y"},{"issue":"3","key":"e_1_2_10_7_1","first-page":"298","article-title":"Self\u2010Sampling in Human Papillomavirus Screening During and Post\u2010COVID\u201019 Pandemic","volume":"76","author":"Tan C. S.","year":"2021","journal-title":"Medical Journal of Malaysia"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.22452\/mjlis.vol25no2.5"},{"key":"e_1_2_10_9_1","unstructured":"A. R.Jerip \u201cLaunching of Program SUARA_ Sarawak Urban and Rural Action for Cervical Cancer Prevention.\u201dUNIMAS Gazette 2023."},{"key":"e_1_2_10_10_1","volume-title":"WHO Guideline For Screening and Treatment of Cervical Pre\u2010cancer Lesions For Cervical Cancer Prevention","year":"2021"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1214\/aoms\/1177731363"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02486-20"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.09.008"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238417"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2021.22.9.2969"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgph.0001793"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.04.007"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-04-0455"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01399.25"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofaa466"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26005"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.71101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.71101","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.71101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T11:27:20Z","timestamp":1787657240000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.71101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":20,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jmv.71101"],"URL":"https:\/\/doi.org\/10.1002\/jmv.71101","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71101"}}