{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T11:22:04Z","timestamp":1787656924822,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Molecular point\u2010of\u2010care testing (POCT) platforms have demonstrated considerable utility in rapid and accurate influenza diagnostics. Among them, the Cepheid Xpert is widely used in clinical practice, while Coyote Flash10 and Genewise NAT\u20103000 were recently approved, with few performance data available. Performances of Flash10 and NAT\u20103000 were evaluated in parallel with the Xpert assay. The limits of detection (LoDs) were determined using serially diluted standard reference materials of inactivated influenza virus A and B (FluA\/B). Clinical performance was assessed using 200 remnant nasopharyngeal swab specimens. Other assay features were also compared. All three platforms featured comparable hands\u2010on procedures and turnaround times ranging from 26 to 33\u2009min. The Xpert assay was more sensitive than the other two, with the lowest LoDs at 72.6, 49.3, 213.2, and 66.8 copies\/mL for FluA H1N1, FluA H3N2, FluB Victoria, and FluB Yamagata, respectively. The Flash10 and NAT\u20103000 assays showed similar LoDs for FluA, ranging from 483.4 to 576.8 copies\/mL; for Victoria and Yamagata, their LoDs were 296.4 and 92.7 copies\/mL for Flash10 and 1419.1 and 591.0 copies\/mL for NAT\u20103000. Clinical performance revealed high concordance across assays. Positive and negative percentage agreements ranged from 93.33% to 100.00%, along with high Kappa values ranging from 0.94 to 0.95. Linear regression analysis showed moderate to strong associations between the cycle threshold values of positive specimens. The two new molecular POCT platforms were similarly user\u2010friendly and showed comparable performance to the Xpert platform for FluA\/B detection, despite their higher LoDs, indicating they are potential alternatives for rapid respiratory virus detection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.71115","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T07:17:48Z","timestamp":1787296668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Performance of Two Molecular Point\u2010of\u2010Care Influenza A\/B Tests Compared to the Cepheid Xpert Xpress Flu\/RSV Assay"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3155-3092","authenticated-orcid":false,"given":"Ran","family":"Zhao","sequence":"first","affiliation":[{"name":"Shanghai Center for Clinical Laboratory Shanghai China"},{"name":"Shanghai Academy of Experimental 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