{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:05:20Z","timestamp":1789664720311,"version":"4.0.1"},"reference-count":14,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A20410"],"award-info":[{"award-number":["U20A20410"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The emergence of diverse SARS\u2010CoV\u20102 Omicron subvariants has reduced the efficacy of existing vaccines, highlighting the need for multivalent vaccines with broader neutralizing capacity. We developed a trivalent adenoviral vector\u2010based vaccine (Ad5\u2010CoV19\u2010prototype\/Delta\/BF.7) encoding the spike proteins of the ancestral strain, Delta variant, and Omicron BF.7 strain, and evaluated its immunogenicity in BALB\/c mice. Four\u2010 to 6\u2010week\u2010old female BALB\/c mice were intramuscularly immunized with 10\u2009\u00b5g of monovalent or trivalent vaccines using single\u2010dose or two\u2010dose (2\u2010week interval) regimens. Neutralizing antibody (nAb) titers against the ancestral strain, Omicron BA.5, and XBB.1.5, as well as IFN\u2010\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses, were measured. The trivalent vaccine elicited significantly higher nAb titers against the ancestral strain (GMT 2183.6 at week 8) and Omicron subvariants (BA.5: GMT 680.0; XBB.1.5: GMT 161.3) after two doses compared to monovalent vaccines. Two doses also enhanced IFN\u2010\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). These results demonstrate that the trivalent vaccine induces enhanced immunogenicity and cross\u2010neutralizing activity, including improved cross\u2010neutralizing antibody titers and IFN\u2010\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses against multiple SARS\u2010CoV\u20102 variants compared to monovalent formulations. These findings suggest potential for broader immune coverage against antigenically drifted variants, although protective efficacy remains to be confirmed in viral challenge models and further clinical evaluation.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71119","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:35:51Z","timestamp":1787654151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced Immunogenicity and Cross\u2010Neutralizing Activity of a Trivalent Adenoviral Vector\u2010Based SARS\u2010CoV\u20102 Vaccine in Mice"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-4699","authenticated-orcid":false,"given":"Jianhua","family":"Li","sequence":"first","affiliation":[{"name":"Departmentof Microbiology, Zhejiang Key Laboratory of Public Health Detection and Pathogenesis Research Zhejiang Provincial Center for Disease Control and Prevention Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchen","family":"Song","sequence":"additional","affiliation":[{"name":"Jiaxing Center for Disease Control and Prevention Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxuan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College Zhejiang Shuren University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruofan","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Information Engineering Zhejiang Chinese Medical University Hangzhou China"},{"name":"School of Public Health, Hangzhou Medical College Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Information Engineering Zhejiang Chinese Medical University Hangzhou China"},{"name":"School of Public Health, Hangzhou Medical College Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Departmentof Microbiology, Zhejiang Key Laboratory of Public Health Detection and Pathogenesis Research Zhejiang Provincial Center for Disease Control and Prevention Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonglan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of the Center for Basic and Translational Research The Second Affiliated Hospital of Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9469-0991","authenticated-orcid":false,"given":"Keda","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Information Engineering Zhejiang Chinese Medical University Hangzhou China"},{"name":"School of Public Health, Hangzhou Medical College Hangzhou 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