{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:38:54Z","timestamp":1792618734457,"version":"4.5.0"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This retrospective single\u2010center study compared nucleic acid testing (NAT), time\u2010resolved fluorescence immunoassay (TRFIA), and enzyme\u2010linked immunosorbent assay (ELISA) for screening hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and\n                    <jats:italic>Treponema<\/jats:italic>\n                    pallidum (TP) among 1243 preoperative and pre\u2010transfusion patients. Specimens were detected by pathogen\u2010targeted NAT, serological TRFIA and routine ELISA panels, respectively. HBV positive rates were 2.17% (NAT), 2.09% (TRFIA) and 1.53% (ELISA); HCV positive rates were 0.08%, 0.24% and 0.08%, without statistical intergroup differences (all\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). TRFIA yielded a significantly higher TP positive rate than ELISA (0.88% versus 0.40%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), while no HIV\u2010positive specimens were identified in this cohort. Kappa tests showed moderate\u2010to\u2010good consistency for HBV (0.711\u20130.842) and perfect concordance between NAT and ELISA for HCV (Kappa\u2009=\u20091.000). Twenty\u2010three discordant samples were found, including eight NAT\u2010only HBV positives suggestive of potential window\u2010period infections and six TRFIA\u2010only TP positives. Limited by an obstetric\u2010female\u2010dominated cohort, scarce HCV cases, and zero HIV positives, standard diagnostic accuracy metrics could not be comprehensively evaluated. NAT and TRFIA exhibit complementary screening values, yet their combined application may increase false\u2010positive risks and lacks systematic cost\u2010effectiveness evidence to support large\u2010scale clinical implementation.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71125","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:40:31Z","timestamp":1788428431000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of NAT, TRFIA, and ELISA for Screening Blood\u2010Borne Pathogens in Preoperative and Pre\u2010Transfusion Patients"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4495-5603","authenticated-orcid":false,"given":"Juan","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengying","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaya","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8774-0471","authenticated-orcid":false,"given":"Zhuo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Xi'an Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina61122153"},{"key":"e_1_2_9_3_1","first-page":"207","article-title":"Individual Donor Nucleic Acid Testing for Blood Safety Against HIV\u20101 and Hepatitis B and C Viruses in a Tertiary Care Hospital","volume":"25","author":"Chatterjee K.","year":"2012","journal-title":"National Medical Journal of India"},{"key":"e_1_2_9_4_1","first-page":"977","article-title":"Nucleic Acid Testing in Colombian Blood Banks","volume":"120","author":"Berm\u00fadez\u2010Forero M. I.","year":"2018","journal-title":"Vox Sanguinis"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.htct.2024.04.120"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-03506-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e37905"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-016-1925-6"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmmb.2021.12.014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2024.241042"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2011.01506.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.13592"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.55133\/eji.310206"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14180"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanwpc.2024.101193"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/lmad078"},{"key":"e_1_2_9_17_1","first-page":"38","article-title":"Cost\u2010Effectiveness Analysis of Hepatitis B and C Nucleic Acid Screening Among Hospitalized Patients in China","volume":"46","author":"Shu S.","year":"2023","journal-title":"Chinese Journal of Laboratory Medicine"},{"key":"e_1_2_9_18_1","article-title":"Added Value of Nucleic Acid Testing in Blood Banks: A 15\u2010Year Retrospective Study From India","volume":"17","author":"Pathak S.","year":"2025","journal-title":"Cureus"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.71125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.71125","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.71125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:57:53Z","timestamp":1792616273000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.71125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":17,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/jmv.71125"],"URL":"https:\/\/doi.org\/10.1002\/jmv.71125","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71125"}}