{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:38:47Z","timestamp":1792618727857,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca Corrente Linea 1"],"award-info":[{"award-number":["Ricerca Corrente Linea 1"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["5\u00d71000 program (2022)"],"award-info":[{"award-number":["5\u00d71000 program (2022)"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>During 2023, dengue virus (DENV) autochthonous circulation was detected in Lazio, Italy. To investigate the magnitude of the phenomenon, anti\u2010DENV serological status of blood donors residing in previously affected Rome municipalities was evaluated. Between December 2024 and April 2025, blood donors consecutively accessing to the seven recruited transfusion medicine units in the Lazio region (Italy) were screened for enrollment. Anti\u2010DENV IgG antibodies were assessed using a commercial ELISA assay (EUROIMMUN, Germany), and positive results were confirmed by virus neutralization (VNT). A total of 1297 consecutive blood donors were enrolled. None reported having a previous diagnosis of dengue. Six came from endemic countries. Initial ELISA screening identified 22 samples (18 positive and 4 equivocal) that were further investigated by VNT. Anti\u2010DENV neutralizing antibodies were confirmed in 7 donors, with a 0.54% prevalence rate (7\/1297; 95% CI 0.14\u20130.94). Only 1 out of the 7 donors with confirmed presence of anti\u2010DENV IgG denied a history of travels to endemic areas. The prevalence of donors with possible local transmission was 0.08% (95% CI: 0.01\u20130.44). The study suggests that undetected viral circulation in the Lazio region is very unlikely, and the rate of mild or asymptomatic infections may be lower than that reported in endemic countries.<\/jats:p>","DOI":"10.1002\/jmv.71147","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:10:40Z","timestamp":1789485040000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dengue Seroprevalence Among Blood Donors in the Lazio Region (Italy): Is There an Iceberg Beneath the Surface?"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0410-9578","authenticated-orcid":false,"given":"Martina","family":"Spaziante","sequence":"first","affiliation":[{"name":"Regional Service for Surveillance and Control of Infectious Diseases (SERESMI)\u2010Lazio Region, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-6080","authenticated-orcid":false,"given":"Luigi","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Colavita","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Mussetto","sequence":"additional","affiliation":[{"name":"Regional Service for Surveillance and Control of Infectious Diseases (SERESMI)\u2010Lazio Region, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3972-8301","authenticated-orcid":false,"given":"Gabriella","family":"De Carli","sequence":"additional","affiliation":[{"name":"Regional Service for Surveillance and Control of Infectious Diseases (SERESMI)\u2010Lazio Region, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Vantaggio","sequence":"additional","affiliation":[{"name":"Regional Service for Surveillance and Control of Infectious Diseases (SERESMI)\u2010Lazio Region, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Agresta","sequence":"additional","affiliation":[{"name":"Regional Service for Surveillance and Control of Infectious Diseases (SERESMI)\u2010Lazio Region, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Coen","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9748-5340","authenticated-orcid":false,"given":"Silvia","family":"Cammisa","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5160-9743","authenticated-orcid":false,"given":"Alessandra","family":"D'Abramo","sequence":"additional","affiliation":[{"name":"High Intensity of Treatment Infectious Diseases Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Maffongelli","sequence":"additional","affiliation":[{"name":"High Intensity of Treatment Infectious Diseases Unit, National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Vaglio","sequence":"additional","affiliation":[{"name":"Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Iaboni","sequence":"additional","affiliation":[{"name":"Immunohematology and Transfusion Medicine Unit, Sant'Andrea University Hospital Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9344-6536","authenticated-orcid":false,"given":"Ida","family":"Casorelli","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Unit, Regina Elena National Cancer Institute IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8972-8343","authenticated-orcid":false,"given":"Luca","family":"Mele","sequence":"additional","affiliation":[{"name":"UOC Servizio Immunotrasfusionale SIMT ASL Roma 3 \u2013 Ospedale G.B. 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