{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:38:43Z","timestamp":1792618723659,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ebola virus is a highly virulent pathogen causing severe hemorrhagic fever with 25%\u201390% mortality. The 2014 outbreak infected over 28,000 people, causing 11,325 deaths. Although there is one FDA\u2010approved vaccine available for limited use, additional vaccines are needed. We developed adenoviral\u2010vectored vaccines expressing the ebolavirus glycoprotein (GP), the sole viral surface protein and primary immunogen. Vaccine constructs spanned three species and five human adenovirus subtypes, including low\u2010seroprevalent serotypes to minimize pre\u2010existing immunity. We compared single\u2010dose and prime\u2010boost regimens, intramuscular versus needle\u2010free intranasal delivery, and serotype\u2010switching strategies. Immunogenicity was assessed by quantifying GP\u2010specific antibodies and T cell responses in BALB\/c mice. Species C vectors (Ad5 and Ad6) elicited strong antibody responses and promoted rapid class switching to IgG compared to species B and D vectors. Serotype\u2010switched regimens further enhanced antibody levels via intramuscular or intranasal routes and generated robust T cell responses when delivered intramuscularly. Intranasal vaccination induced detectable IgA but no measurable T cell responses, indicating route\u2010dependent immune compartmentalization. Sera partially inhibited viral entry in a dose\u2010dependent manner, with the greatest reduction observed in the Ad5\/Ad6 regimen. Collectively, adenoviral species selection, serotype switching, and route of administration significantly influence GP\u2010specific immunity and warrant further evaluation in protective challenge models.<\/jats:p>","DOI":"10.1002\/jmv.71161","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:53:35Z","timestamp":1789991615000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Systemic and Mucosal Immune Responses From Serotype\u2010Switched Viral\u2010Vectored Ebola Vaccines"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8574-5073","authenticated-orcid":false,"given":"Joshua","family":"Wiggins","sequence":"first","affiliation":[{"name":"Nebraska Center for Virology University of Nebraska\u2014Lincoln Lincoln Nebraska USA"},{"name":"School of Biological Sciences University of Nebraska\u2014Lincoln Lincoln Nebraska 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