{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:55:50Z","timestamp":1792418150028,"version":"4.4.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360001"],"award-info":[{"award-number":["82360001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82660002"],"award-info":[{"award-number":["82660002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease, but the contribution of long non\u2010coding RNAs (lncRNAs) to RSV\u2010induced epithelial\u2013mesenchymal transition (EMT)\u2010associated changes remains unclear. We used transcriptomic screening in RSV\u2010exposed BEAS\u20102B cells to identify candidate lncRNAs and then examined SRGAP2\u2010AS1 in cell and mouse models. Expression was measured by RT\u2010qPCR, and loss\u2010 and gain\u2010of\u2010function experiments were combined with RNA immunoprecipitation, co\u2010immunoprecipitation, Western blot analysis, and TCID\n                    <jats:sub>50<\/jats:sub>\n                    assays. SRGAP2\u2010AS1 was increased in RSV\u2010infected BEAS\u20102B cells and mouse lung tissue. Silencing SRGAP2\u2010AS1 reduced viral replication, lowered IL\u20106 and IL\u20101\u03b2 mRNA, increased IFN\u2010\u03b2 mRNA, and attenuated EMT\u2010associated marker changes in RSV\u2010infected BEAS\u20102B cells. SRGAP2\u2010AS1 was predominantly cytoplasmic and enriched in PTBP1 immunoprecipitates. Reciprocal co\u2010immunoprecipitation showed that PTBP1 and ZEB1 were present in the same endogenous complex, and ZEB1 abundance changed with SRGAP2\u2010AS1 gain and loss of function. ZEB1 knockdown produced parallel changes in viral RNA, immune\u2010related transcripts, and EMT\u2010associated markers. Ectopic ZEB1 expression reversed the E\u2010cadherin change caused by SRGAP2\u2010AS1 depletion, supporting a downstream role for ZEB1 in the epithelial arm of the EMT\u2010associated phenotype. These findings support regulatory relationships among SRGAP2\u2010AS1, PTBP1, and ZEB1 during RSV infection. The causal order of the viral and immune\u2010related changes, and the in vivo relevance of the EMT\u2010associated phenotype, remain unresolved.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71166","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:31:46Z","timestamp":1790602306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["SRGAP2\u2010AS1 Regulates RSV Replication and EMT\u2010Associated Changes Through PTBP1 and ZEB1"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4406-6060","authenticated-orcid":false,"given":"Xinran","family":"Tan","sequence":"first","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"},{"name":"Department of Pain Medicine Affiliated Hospital of Zunyi Medical University Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Wu","sequence":"additional","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"},{"name":"Department of Critical Care Medicine The Second People's Hospital of Guiyang (Jinyang Hospital)\/The Affiliated Jinyang Hospital of Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Gao","sequence":"additional","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Li","sequence":"additional","affiliation":[{"name":"The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiqiang","family":"He","sequence":"additional","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Guizhou Provincial People's Hospital Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Peng","sequence":"additional","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8889-2909","authenticated-orcid":false,"given":"Daishun","family":"Liu","sequence":"additional","affiliation":[{"name":"Zunyi Medical University Zunyi Guizhou China"},{"name":"Department of Respiratory and Critical Care Medicine Guizhou Provincial People's Hospital Guiyang Guizhou China"},{"name":"NHC Key Laboratory of Pulmonary Immunological Diseases Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00919-w"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00010-16"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2020.4641"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-020-00781-5"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2022.998296"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3289-4"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01507"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01675"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.08.007"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.875691"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1110774"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0151-6"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00260"},{"issue":"3","key":"e_1_2_12_15_1","first-page":"4138","article-title":"LncRNA MEG3 Ameliorates Respiratory Syncytial Virus Infection by Suppressing TLR4 Signaling","volume":"17","author":"Tao X. 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