{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:56:11Z","timestamp":1792418171177,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      A substantial proportion of cervical intraepithelial neoplasia grade 2 (CIN2) spontaneously regress, supporting the use of active surveillance over immediate treatment. Identification of prognostic factors associated with higher rates of spontaneous regression could enable better selection of patients in whom such approach would be safe. We evaluated the prognostic value of\n                      <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic>\n                      methylation status, HPV genotype, and baseline cytology for predicting spontaneous regression of CIN2. Women aged 18\u201340 years with CIN2 were prospectively enroled and followed for 2 years. At baseline, HPV genotyping was performed together with\n                      <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic>\n                      methylation analysis. Among 119 enroled patients, the rate of spontaneous regression of CIN2 was 61.3% (73\/119). Regression of CIN2 was significantly associated with HPV genotype (\n                      <jats:italic>\u03c7<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u200919.374;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002) and negative methylation result (\n                      <jats:italic>\u03c7<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u200910.945;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.012). Negative methylation in combination with low\u2010grade cytology and non\u2010HPV16 genotype was associated with regression rates of 71.4% and 70.4%, respectively. Spontaneous regression of CIN2 is more frequent in women with non\u2010HPV16 infection and negative\n                      <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic>\n                      methylation. A combination of these markers improves identification of women with lower risk of disease progression and supports safer implementation of active surveillance and conservative management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Trial Registration<\/jats:title>\n                    <jats:p>The study was registered at ClinicalTrials.gov as NCT05624827.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.71175","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:33:43Z","timestamp":1791891223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of Methylation Marker\n                    <i>FAM19A4\/mir124\u20102<\/i>\n                    and HPV Genotype in Active Surveillance of Women With Cervical Intraepithelial Neoplasia Grade 2 (CIN2)"],"prefix":"10.1002","volume":"98","author":[{"given":"Milena","family":"Miklu\u0161","sequence":"first","affiliation":[{"name":"University Clinic for Gynaecology and Obstetrics, University Medical Centre Maribor Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3216-7564","authenticated-orcid":false,"given":"Mario","family":"Poljak","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine University of Ljubljana Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Mlakar","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine University of Ljubljana Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrej","family":"Cokan","sequence":"additional","affiliation":[{"name":"University Clinic for Gynaecology and Obstetrics, University Medical Centre Maribor Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Paki\u017e","sequence":"additional","affiliation":[{"name":"University Clinic for Gynaecology and Obstetrics, University Medical Centre Maribor Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jure","family":"Knez","sequence":"additional","affiliation":[{"name":"University Clinic for Gynaecology and Obstetrics, University Medical Centre Maribor Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyla","family":"Al\u2010Mahdawi","sequence":"additional","affiliation":[{"name":"University Clinic for Gynaecology and Obstetrics, University Medical Centre Maribor Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luka","family":"Ro\u0161kar","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology General Hospital Murska Sobota Murska Sobota Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4208-9683","authenticated-orcid":false,"given":"Mario","family":"Gorenjak","sequence":"additional","affiliation":[{"name":"Centre for Human Molecular Genetics and Pharmacogenomics University of Maribor Faculty of Medicine Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3061-5319","authenticated-orcid":false,"given":"Anja","family":"O\u0161trbenk","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine University of Ljubljana Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grega Gimpelj","family":"Domjani\u010d","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Faculty of Medicine University of Ljubljana Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6959-1836","authenticated-orcid":false,"given":"Andra\u017e","family":"Dovnik","sequence":"additional","affiliation":[{"name":"University Clinic for Gynaecology and Obstetrics, University Medical Centre Maribor Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(22)00191-7"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(24)00524-2"},{"key":"e_1_2_11_4_1","first-page":"CD012847","article-title":"Obstetric Outcomes After Conservative Treatment for Cervical Intraepithelial Lesions and Early Invasive Disease","volume":"11","author":"Kyrgiou M.","year":"2017","journal-title":"Cochrane Database of Systematic 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