{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:50:07Z","timestamp":1792417807788,"version":"4.4.0"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cervical cancer is the fourth most common malignancy in women worldwide. Biomarker\u2010assisted screening may improve diagnostic accuracy, but biomarkers predicting progression of low\u2010grade squamous intraepithelial lesions (LSIL) remain limited. We integrated single\u2010cell RNA sequencing and spatial transcriptomics to identify prognostic biomarkers associated with LSIL outcomes, followed by validation using improved in situ sequencing (IISS). Analysis of 341,978 single cells identified eight major cell populations and twelve epithelial subclusters, including a previously unrecognized subcluster, epi8. Although mainly derived from normal cervix, epi8 exhibited cancer\u2010associated features and shared molecular characteristics with the prognostic biomarkers\n                    <jats:italic>FAM19A4<\/jats:italic>\n                    and\n                    <jats:italic>miR124\u20102<\/jats:italic>\n                    .\n                    <jats:italic>In situ<\/jats:italic>\n                    sequencing showed that epi8 localized adjacent to human papillomavirus\u2010infected basal cells, with stage\u2010dependent spatial changes during disease progression. Retrospective analysis further demonstrated that epi8\u2010associated markers stratified LSIL into progression, persistence, and regression groups, with\n                    <jats:italic>CRYAB<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>high<\/jats:italic>\n                    <\/jats:sup>\n                    <jats:italic>\/GAS5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>high<\/jats:italic>\n                    <\/jats:sup>\n                    associated with progression and\n                    <jats:italic>RPLP1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>high<\/jats:italic>\n                    <\/jats:sup>\n                    <jats:italic>\/SNHG5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>high<\/jats:italic>\n                    <\/jats:sup>\n                    associated with regression. We identified a previously unrecognized epithelial subcluster associated with early cervical carcinogenesis and demonstrated that its molecular markers enable risk stratification of LSIL, providing candidate biomarkers for precision triage in cervical cancer screening.\n                  <\/jats:p>","DOI":"10.1002\/jmv.71184","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T11:42:30Z","timestamp":1792237350000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating scRNA\u2010Seq and Spatial Transcriptomics Reveal Novel Risk Stratification Signatures in Cervical Carcinoma"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4428-521X","authenticated-orcid":false,"given":"Jiayu","family":"Chen","sequence":"first","affiliation":[{"name":"School of Medicine Huaqiao University Xiamen 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