{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:53:46Z","timestamp":1791280426653,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,5,30]],"date-time":"2002-05-30T00:00:00Z","timestamp":1022716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2002,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous experiments from our laboratory have shown that application of brain\u2010derived neurotrophic factor (BDNF) to the red nucleus or the motor cortex stimulates an increase in the expression of regeneration\u2010associated genes in rubrospinal and corticospinal neurons. Furthermore, we have previously shown that BDNF application stimulates regeneration of rubrospinal axons into a peripheral graft after a thoracic injury. The current study investigates whether application of BDNF to the motor cortex will facilitate regeneration of corticospinal neurons into a peripheral nerve graft placed into the thoracic spinal cord. In adult Sprague Dawley rats, the dorsal columns and the corticospinal tract between T9 and T10 were ablated by suction, and a 5\u2010mm\u2010long segment of predegenerated tibial nerve was autograft implanted into the lesion. With an osmotic pump, BDNF was infused directly into the parenchyma of the motor cortex for 14 days. Growth of the corticospinal tract into the nerve graft was then evaluated by transport of an anterograde tracer. Anterogradely labeled corticospinal fibers were not observed in the peripheral nerve graft in animals treated with saline or BDNF. Serotinergic and noradrenergic fibers, as well as peripheral sensory afferents, were observed to penetrate the graft, indicating the viability of the peripheral nerve graft as a permissive growth substrate for these specific fiber types. Although treatment of the corticospinal fibers with BDNF failed to produce regeneration into the graft, there was a distinct increase in the number of axonal sprouts rostral to the injury site. This indicates that treatment of corticospinal neurons with neurotrophins, e.g., BDNF, can be used to enhance sprouting of corticospinal axons within the spinal cord. Whether such sprouting leads to functional recovery after spinal cord injury is currently under investigation. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10275","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:33:49Z","timestamp":1030318429000},"page":"160-168","source":"Crossref","is-referenced-by-count":78,"title":["Brain\u2010derived neurotrophic factor applied to the motor cortex promotes sprouting of corticospinal fibers but not regeneration into a peripheral nerve transplant"],"prefix":"10.1002","volume":"69","author":[{"given":"G.W.","family":"Hiebert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Khodarahmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"McGraw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.D.","family":"Steeves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Tetzlaff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/296150a0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-09-03556.1999"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7413"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/378498a0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6705"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(93)90157-T"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80681-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90212-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1996.0065"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000219"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.510"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-13-04782.2001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6171034"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/37776"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-14-05810.1999"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1523\/JNEUROSCI.18-02-00779.1998","article-title":"Fetal spinal cord transplants support growth of supraspinal and segmental projections after cervical spinal cord hemisection in the neonatal rat","volume":"18","author":"Diener PS","year":"1998","journal-title":"J Neurosci"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(99)00072-6"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19991129)414:4<495::AID-CNE6>3.0.CO;2-S"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050944"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012705070-6\/50004-4"},{"key":"e_1_2_7_22_1","unstructured":"GiehlKM HammondENL HiebertGW KobayashiNR FernandesKJ MestresP TetzlaffW.2000.Differential effects of BDNF and NT\u20103 on GAP\u201043 Ta1\u2010tubulin and neurofilament\u2010M expression in axotomized rat corticospinal neurons in vivo. 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