{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:11:34Z","timestamp":1791047494789,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T00:00:00Z","timestamp":1034121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2002,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Results of this study indicate a radioprotective effect of peroxiredoxin\u2010I. Peroxiredoxin\u2010I is an antioxidant that scavenges hydroperoxides, whereas reactive oxygen species are the main mediators of ionizing radiation toxicity. We hypothesized that peroxiredoxin\u2010I might be induced by cellular exposure to radiation and act to protect them against its cytotoxic effects. Western blot and Northern blot analyses were used to assess peroxiredoxin\u2010I protein and mRNA expression. Rat C6 glioma cells were engineered to overexpress sense or antisense human peroxiredoxin\u2010I using retroviral vectors. Clonogenic cell survival was used to assess radiosensitivities of the engineered cells. Ionizing radiation induced peroxiredoxin\u2010I protein and mRNA expression in human HT29 colon cancer and rat C6 glioma cells in a dose\u2010 and time\u2010dependent manner over a 24 hr period. To determine the effect of peroxiredoxin\u2010I on radiation responses, C6 glioma cells were engineered to overexpress sense or antisense human peroxiredoxin\u2010I. In clonogenic assays, cells overexpressing peroxiredoxin\u2010I were more radioresistant. Cells transduced with antisense peroxiredoxin\u2010I were marginally more sensitive to radiation toxicity. Irradiation can induce peroxiredoxin\u2010I expression, and the increased peroxiredoxin\u2010I may protect cells from further radiation damage. These results suggest that protection by peroxiredoxin\u2010I may play an important role in the survival of glioma and colon cancer cells in patients undergoing radiation therapy. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10435","type":"journal-article","created":{"date-parts":[[2002,11,21]],"date-time":"2002-11-21T18:08:16Z","timestamp":1037902096000},"page":"794-798","source":"Crossref","is-referenced-by-count":48,"title":["Induction of radioprotective peroxiredoxin\u2010I by ionizing irradiation"],"prefix":"10.1002","volume":"70","author":[{"given":"Wen\u2010Cheng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H.","family":"McBride","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke S.","family":"Iwamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad 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