{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T13:14:13Z","timestamp":1747746853029},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,10,18]],"date-time":"2002-10-18T00:00:00Z","timestamp":1034899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2003,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochemistry and nitric oxide synthase (NOS) immunocytochemistry combined with radioassay of calcium\u2010dependent NOS activity, we examined the occurrence of NADPHd staining and NOS immunoreactivity (NOS\u2010IR) in the dorsal root ganglia (DRG) neurons, dorsal root afferents, and axons projecting via gracile fascicle to gracile nucleus 14 days after unilateral sciatic nerve transection in the rabbit. Mild to moderate NADPHd staining and NOS\u2010IR appeared in a large number of small and medium\u2010sized to large neurons in the ipsilateral L4\u2013L6 DRG, accompanied by enhanced NOS\u2010IR of thick myelinated fibers in the ipsilateral L4\u2013L6 dorsal roots. A noticeable increase in the density of punctate NADPHd staining occurred throughout laminae I\u2013IV in the ipsilateral medial part of the dorsal horn in L4\u2013L6 segments. Concurrently, a statistically significant decrease in the number of small NADPHd\u2010exhibiting neurons in laminae I\u2013II and, in contrast to this, a statistically significant increase of medium\u2010sized to large NADPHd\u2010stained somata in the ipsilateral laminae III\u2013VI of L4\u2013L6 segments were found. A detailed compartmentalization of L4\u2013L6 segments into gray and white matter regions disclosed substantially increased catalytic NOS activity and inducible NOS mRNA levels in the dorsal horn and dorsal column ipsilaterally to the peripheral injury. A noticeable increase in the number of thick myelinated NADPHd\u2010exhibiting and NOS\u2010IR axons was noted in the ipsilateral gracile fascicle, terminating in dense, punctate NADPHd staining in the neuropil of the gracile nucleus. These observations indicate that the de novo\u2010synthesized NOS in the lesioned primary afferent neurons resulting after sciatic nerve transection may be involved in an increase in NADPHd staining and NOS\u2010IR in the ipsilateral dorsal roots and dorsal horn of L4\u2013L6 segments, whence NOS could be supplied to ascending axons of the gracile fascicle. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10470","type":"journal-article","created":{"date-parts":[[2002,12,28]],"date-time":"2002-12-28T17:03:13Z","timestamp":1041094993000},"page":"300-313","source":"Crossref","is-referenced-by-count":14,"title":["Peripheral axotomy affects nicotinamide adenine dinucleotide phosphate diaphorase and nitric oxide synthases in the spinal cord of the rabbit"],"prefix":"10.1002","volume":"71","author":[{"given":"Nade\u017eda","family":"Luk\u00e1\u010dov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e1\u0161a","family":"\u010c\u00ed\u017ekov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Kri\u017eanov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav","family":"Pavel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Mar\u0161ala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jozef","family":"Mar\u0161ala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903060303"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(93)90316-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.2.682"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/347768a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4471-1305-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(95)00325-V"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02769177"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(93)90161-H"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(94)90147-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90019-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(93)90579-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90528-S"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19990115)55:2<198::AID-JNR7>3.0.CO;2-M"},{"key":"e_1_2_6_16_1","first-page":"281","volume-title":"Hyperalgesia and allodynia","author":"Haley JE","year":"1992"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-200005150-00003"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90257-5"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-03-00893.1989"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(87)90088-7"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(94)90031-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(99)00034-2"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90167-7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1523\/JNEUROSCI.10-06-02026.1990","article-title":"Tachykinins and calcitonin gene\u2010related peptide enhance release of endogenous glutamate and aspartate from the rat spinal dorsal horn slice","volume":"10","author":"Kangrga I","year":"1990","journal-title":"J Neurosci"},{"key":"e_1_2_6_25_1","first-page":"321","article-title":"NADPH diaphorase activity in the primary sensory neurons following peripheral axotomy","volume":"56","author":"Kuch\u00e1rov\u00e1 K","year":"2001","journal-title":"Biologia"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00233-X"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00690-8"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990405)406:2<263::AID-CNE9>3.0.CO;2-4"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(93)90124-8"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00258-3"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90394-H"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01230-9"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4179(87)90015-1"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(89)90077-6"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(90)90080-W"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(90)90696-7"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490350108"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490370506"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-11-07633.1995"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00065-4"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90608-4"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(83)90085-7"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050150"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)91456-3"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00238861"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90361-1"},{"key":"e_1_2_6_47_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.55782\/ane-1996-1106","article-title":"Biphasic enhancement of nitric oxide synthase activity and cGMP level following brain ischemia in gerbils","volume":"56","author":"Strosznajder J","year":"1996","journal-title":"Acta Neurobiol Exp"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00392-4"},{"key":"e_1_2_6_49_1","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1523\/JNEUROSCI.09-04-01303.1989","article-title":"Axonal transport and localization of B\u201050\/GAP\u201043\u2010like immunoreactivity in regenerating sciatic and facial nerves of the rat","volume":"9","author":"Tetzlaff W","year":"1989","journal-title":"J Neurosci"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903210204"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90411-9"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91530-6"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903390107"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(97)00025-8"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(91)90093-9"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)90206-O"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-0597-0"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004691-199701000-00002"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.14.7675"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.2000"},{"key":"e_1_2_6_61_1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1523\/JNEUROSCI.11-02-00543.1991","article-title":"NGF rescues substance P expression but not neurofilament or tubulin gene expression in axotomized sensory neurons","volume":"11","author":"Wong J","year":"1991","journal-title":"J Neurosci"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)84619-7"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903350408"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.10470","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T16:34:23Z","timestamp":1704731663000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.10470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12,24]]},"references-count":62,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,2,15]]}},"alternative-id":["10.1002\/jnr.10470"],"URL":"https:\/\/doi.org\/10.1002\/jnr.10470","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12,24]]}}}