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Estrogen induces internalization of MOR in cell groups of the limbic\u2010hypothalamic lordosis\u2010regulating circuit. Because rapid MOR internalization is mediated by estrogen release of endogenous opioid peptides, internalization has been used as a neurochemical signature of estrogen action in the central nervous system. Together these results indicate that estrogen induces a MOR mediated inhibition of sexual receptivity. To determine which estrogen receptor, estrogen receptor\u2010\u03b1 (ER\u03b1) or estrogen receptor\u2010\u03b2 (ER\u03b2), mediates MOR internalization, ER\u03b1 knockout (ER\u03b1KO), ER\u03b2 knockout (ER\u03b2KO) and wild\u2010type (WT) mice were used in the present study. WT, ER\u03b1KO and ER\u03b2KO mice had similar MOR distributions in the limbic\u2010hypothalamic lordosis\u2010regulating circuit. Estrogen treatment internalized MOR in the medial preoptic nucleus of ovariectomized WT and ER\u03b2KO, but not ER\u03b1KO mice. Treatment of ER\u03b1KO mice with the selective endogenous MOR ligand, endomorphin\u20101, induced levels of MOR internalization similar to WT mice suggesting that MOR in ER\u03b1KO mice could be activated and were probably functional. The results of the present experiments indicate that ER\u03b1 is required for estrogen\u2010induced MOR internalization and suggest that ER\u03b1 can mediate rapid actions of estrogen. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10526","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T23:07:03Z","timestamp":1048028823000},"page":"802-810","source":"Crossref","is-referenced-by-count":84,"title":["Estrogen receptor\u2010\u03b1 is required for estrogen\u2010induced \u03bc\u2010opioid receptor internalization"],"prefix":"10.1002","volume":"71","author":[{"given":"Paul E","family":"Micevych","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie F.","family":"Rissman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan\u2010\u00c5ke","family":"Gustafsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Sinchak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490280307"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"5921","DOI":"10.1523\/JNEUROSCI.17-15-05921.1997","article-title":"Noxious cutaneous thermal stimuli induce a graded release of endogenous substance P in the spinal cord: Imaging peptide action in vivo","volume":"17","author":"Allen BJ","year":"1997","journal-title":"J Neurosci"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(72)90029-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0230929"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.9.11.1441"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1523\/JNEUROSCI.18-03-01056.1998","article-title":"Interrelationships between somatostatin sst2A receptors and somatostatin\u2010containing axons in rat brain: evidence for regulation of cell surface receptors by endogenous somatostatin","volume":"18","author":"Dournaud P","year":"1998","journal-title":"J Neurosci"},{"key":"e_1_2_6_8_1","unstructured":"EckersellCB MicevychPE.2003.Distribution of \u03bc\u2010 and \u03b4\u2010opioid receptors with respect to CCK immunoreactivity in the hypothalamus and limbic system. 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