{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:01:37Z","timestamp":1760954497500},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,4,10]],"date-time":"2003-04-10T00:00:00Z","timestamp":1049932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ontario Mental Health Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reports of elevated Gs \u03b1 subunit (\u03b1<jats:sub>s<\/jats:sub>) immunolabeling and cAMP\u2010mediated hyper\u2010functionality in autopsied cerebral cortical brain regions from bipolar affective disorder (BD) patients suggest signal transduction abnormalities occur in this disorder. Because covalent modification of \u03b1<jats:sub>s<\/jats:sub> can affect its turnover and levels, we determined whether CTX\u2010catalyzed and endogenous [<jats:sup>32<\/jats:sup>P] adenosine diphosphate (ADP)\u2010ribosylation of \u03b1<jats:sub>s<\/jats:sub> isoforms are altered in temporal and occipital cortical regions, which show elevated \u03b1<jats:sub>s<\/jats:sub> levels in BD as compared to nonpsychiatric subjects. Reduced CTX\u2010catalyzed [<jats:sup>32<\/jats:sup>P]ADP\u2010ribosylated \u03b1<jats:sub>s\u2010S<\/jats:sub> and endogenous [<jats:sup>32<\/jats:sup>P]ADP\u2010ribosylation of a 39\u2010kDa \u03b1<jats:sub>s<\/jats:sub>\u2010like protein were found in BD temporal cortex compared to controls. These findings suggest that clearance of these \u03b1<jats:sub>s<\/jats:sub> isoforms through ADP\u2010ribosylation may be decreased in BD temporal cortex. Although no differences were observed in mean levels of endogenous and CTX\u2010catalyzed [<jats:sup>32<\/jats:sup>P]ADP\u2010ribosylation of \u03b1<jats:sub>s\u2010L<\/jats:sub> in BD temporal cortex, \u03b1<jats:sub>s\u2010L<\/jats:sub> immunolabeling was elevated significantly and correlated inversely with the degree of endogenous [<jats:sup>32<\/jats:sup>P]ADP\u2010ribosylation of this subunit. In addition, endogenous [<jats:sup>32<\/jats:sup>P]ADP\u2010ribosylation of an exogenous substrate, myelin basic protein, was similar in BD and comparison subject temporal cortex. Taken together, these observations suggest that elevations of \u03b1<jats:sub>s<\/jats:sub> in BD brain are more likely related to factors affecting the disposition or availability of \u03b1<jats:sub>s<\/jats:sub> to this posttranslational enzymatic modification. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10620","type":"journal-article","created":{"date-parts":[[2003,5,12]],"date-time":"2003-05-12T11:32:32Z","timestamp":1052739152000},"page":"638-645","source":"Crossref","is-referenced-by-count":2,"title":["Altered CTX\u2010catalyzed and endogenous [<sup>32<\/sup>P]ADP\u2010ribosylation of stimulatory G protein \u03b1<sub>s<\/sub> isoforms in postmortem bipolar affective disorder temporal cortex"],"prefix":"10.1002","volume":"72","author":[{"given":"Stavroula","family":"Andreopoulos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter 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