{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:18:28Z","timestamp":1791109108701,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,5,23]],"date-time":"2003-05-23T00:00:00Z","timestamp":1053648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2003,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Presenilin 1 interacts with \u03b2\u2010catenin, an essential component of the Wnt signaling pathway. To elucidate the role of presenilin 1\u2010\u03b2\u2010catenin interaction in neuronal differentiation, we established SH\u2010SY5Y cells stably expressing wild\u2010type presenilin 1, P117L mutant presenilin 1, which is linked to the early\u2010onset familial form of Alzheimer's disease, and D385A mutant presenilin 1, which has no aspartyl proteinase activity. We demonstrate that SH\u2010SY5Y cells stably expressing D385A mutant presenilin 1 failed to differentiate in response to retinoic acid treatment. Retinoic acid caused an increase in nuclear \u03b2\u2010catenin levels in SH\u2010SY5Y cells, which was followed by an increase in cyclin D1 protein levels. Abnormal cellular accumulation of \u03b2\u2010catenin was observed in D385A mutant transfected cells, whereas nuclear \u03b2\u2010catenin and cellular cyclin D1 levels failed to increase. Conversely, SH\u2010SY5Y cells expressing the P117L mutant differentiated normally and showed increased nuclear \u03b2\u2010catenin and cellular cyclin D1 levels. These findings suggest that neuronal differentiation of SH\u2010SY5Y cells involves the Wnt signaling pathway and that presenilin 1 plays a crucial role in Wnt signal transduction by regulating the nuclear translocation of \u03b2\u2010catenin. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10641","type":"journal-article","created":{"date-parts":[[2004,10,22]],"date-time":"2004-10-22T01:05:23Z","timestamp":1098407123000},"page":"166-175","source":"Crossref","is-referenced-by-count":39,"title":["Presenilin 1 mediates retinoic acid\u2010induced differentiation of SH\u2010SY5Y cells through facilitation of Wnt 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