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We demonstrate the expression of all four PAR subtypes in the postnatal eye and in retina of the adult rat by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR). PAR\u20101 is regulated developmentally in the eye, with a decrease from P1, P9, to P16, whereas levels for PAR\u20102, PAR\u20103, and PAR\u20104 remain unchanged throughout. In the retina of the adult rat, PAR\u20101 is highly expressed, whereas PAR\u20102 and PAR\u20103 are moderately expressed, compared to low PAR\u20104 expression. To elucidate possible roles of PARs after trauma, we carried out semiquantitative RT\u2010PCR analysis of expression of all 4 PAR subtypes, beginning 6 hr after partial optic nerve crush (ONC) in the adult rat until 3 weeks after the mild trauma. Levels of PAR mRNA for all four subtypes were upregulated as early as 6 hr after unilateral ONC, except PAR\u20103, which showed a delayed upregulation. PAR\u20101, PAR\u20103, and PAR\u20104 mRNA levels returned to almost basal levels at 3 weeks post\u2010crush, whereas PAR\u20102 mRNA level was still high by the end of 3 weeks after crush. Although the lesion was unilateral, PAR mRNA expression in the contralateral, uninjured side was affected to levels almost comparable to those in the injured side. Previous studies have shown an increase in thrombin levels at the site of injury, retinal ganglion cell degeneration by necrosis and apoptosis, and PAR activation as consequences of nerve crush. PAR upregulation because of nerve crush in the mild trauma model could act as an effector of early cell death. Eventual return of receptor mRNA to basal levels is consistent with neuroprotection. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10643","type":"journal-article","created":{"date-parts":[[2004,10,22]],"date-time":"2004-10-22T01:05:23Z","timestamp":1098407123000},"page":"246-254","source":"Crossref","is-referenced-by-count":25,"title":["Protease\u2010activated receptor subtype expression in developing eye and adult retina of the rat after optic nerve 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