{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T19:41:09Z","timestamp":1780083669762,"version":"3.54.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,7,22]],"date-time":"2003-07-22T00:00:00Z","timestamp":1058832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2003,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Optic nerve injury leads to retinal ganglion cell apoptosis, thus preventing fiber regeneration. Peripheral nerve grafts are known to promote survival and regeneration in injured adult mammalian central nervous system, including optic nerve, but the mechanisms of their activity remain unclear. It is likely that they attenuate the apoptotic cascade triggered by axotomy in retinal ganglion cells. The aim of this work was to examine the role of the antiapoptotic gene bcl\u20102 in the optic nerve regeneration induced by such grafts. Experiments were carried out on bcl\u20102\u2010deficient and wild\u2010type mice. We have reported previously that predegeneration markedly enhances neurotrophic activity of peripheral nerve grafts, so we applied both predegenerated and non\u2010predegenerated implants to the transected optic nerves. We studied the neurotrophic effects of bcl\u20102\u2010deficient grafts on wild\u2010type and bcl\u20102 knock\u2010out optic nerves, as well as wild\u2010type grafts on both strains of mouse optic nerves. After application of fluorescent dye to the end of the graft, we counted the stained retinal ganglion cells. Predegenerated wild\u2010type grafts promoted survival and outgrowth of retinal ganglion cells axons in both types of mice. By contrast, non\u2010predegenerated and predegenerated bcl\u20102\u2010deficient grafts induced little or no regeneration in the optic nerves. These results indicate that the lack of bcl\u20102 gene does not deprive retinal ganglion cells of their regenerative potential. At the same time, we found that bcl\u20102 knock\u2010out dispossesses peripheral nerves of their neurotrophic activity. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.10708","type":"journal-article","created":{"date-parts":[[2003,8,27]],"date-time":"2003-08-27T13:42:57Z","timestamp":1061991777000},"page":"846-852","source":"Crossref","is-referenced-by-count":8,"title":["Bcl\u20102 deficiency deprives peripheral nerves of neurotrophic activity against injured optic nerve"],"prefix":"10.1002","volume":"73","author":[{"given":"Katarzyna","family":"Kotulska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Lewin\u2010Kowalik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Larysz\u2010Brysz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wies\u0142aw","family":"Marcol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbigniew","family":"Fus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01637-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.01962.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1523\/JNEUROSCI.09-04-01263.1989","article-title":"Effect of NGF on the survival of rat retinal ganglion cells following optic nerve section","volume":"9","author":"Carmignoto G","year":"1989","journal-title":"J Neurosci"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199904060-00034"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01317.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(99)00032-7"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/385434a0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400431"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-19-08367.1999"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(99)00240-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-23-09172.1997"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpd.2000.106903"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200110290-00041"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00230531"},{"key":"e_1_2_6_16_1","first-page":"24","article-title":"Optic nerve regeneration by peripheral nerve transplant","volume":"13","author":"Fukuda Y","year":"1990","journal-title":"Neurosci Res"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480250408"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.23.1.579"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7540"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01352-6"},{"key":"e_1_2_6_21_1","first-page":"23","article-title":"Axonal regeneration of mouse retinal ganglion cells by peripheral nerve transplantation; 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