{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:52:31Z","timestamp":1790553151832,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,4,18]],"date-time":"2001-04-18T00:00:00Z","timestamp":987552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) are glucocorticoid\u2010activated transcription factors essential for maintenance of cellular homeostasis. Differential activation of these adrenocorticosteroid receptors (ACR) is thought to influence neuronal viability, particularly under challenging cellular conditions. The present study is designed to determine the effects of receptor blockade and excitotoxic insult on MR and GR mRNA expression and neuronal viability in hippocampus. Male Sprague\u2013Dawley rats were pretreated for 48 hr with vehicle, MR antagonist spironolactone (SPIRO) (50 mg\/kg, twice daily, s.c.), or GR antagonist RU486 (25 mg\/kg, twice daily, s.c.) and subsequently injected with saline or the glutamate analog kainic acid (KA) (12 mg\/kg i.p.). Twenty\u2010four hr post\u2010insult, MR and GR mRNA levels were assessed by in situ hybridization analysis, and hippocampal neurons were counted to assess KA\u2010induced cell loss. MR blockade with SPIRO increased basal MR mRNA levels in hippocampal subregions CA1, CA3, and dentate gyrus (DG) and increased basal GR mRNA levels in CA3. GR blockade with RU486 increased basal GR mRNA levels in CA3. The excitotoxin KA decreased MR mRNA levels in CA1 and CA3, decreased GR mRNA levels in DG, and negated all antagonist\u2010induced increases of ACR mRNAs. Cell counts quantifying KA damage indicated increased CA3 vulnerability to KA insult after treatment with MR antagonist spironolactone but demonstrated no significant cell loss in any other group or region. These results demonstrate dynamic regulation of hippocampal MR and GR mRNAs after ACR antagonist treatment and kainate toxicity, underscoring the potential importance of MR and GR availability to neuronal viability after insult. J. Neurosci. Res. 64:277\u2013283, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.1076","type":"journal-article","created":{"date-parts":[[2004,10,28]],"date-time":"2004-10-28T11:11:55Z","timestamp":1098961915000},"page":"277-283","source":"Crossref","is-referenced-by-count":19,"title":["Adrenocorticosteroid receptor blockade and excitotoxic challenge regulate adrenocorticosteroid receptor mRNA levels in hippocampus"],"prefix":"10.1002","volume":"64","author":[{"given":"Deanna L.","family":"McCullers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Herman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,4,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2781282"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(84)90154-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1210\/endo.139.4.5896","article-title":"Regulation of glucocorticoid receptor and mineralocorticoid receptor messenger ribonucleic acids by selective agonists in the rat hippocampus","volume":"139","author":"Chao HM","year":"1998","journal-title":"Endocrinology"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1210\/endo.130.1.1309341","article-title":"Spironolactone, an aldosterone antagonist, acts as an antiglucocorticosteroid on the mouse mammary tumor virus promoter","volume":"130","author":"Couette B","year":"1992","journal-title":"Endocrinology"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90245-I"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1996.66051836.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-3-11-1886"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-18-07462.1998"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1993.1022"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.12.4495"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90345-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91069-3"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.18.8527"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199809140-00031"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1986.66.4.1121"},{"key":"e_1_2_6_17_1","first-page":"873","article-title":"Glucocorticoids may enhance oxygen radical\u2010mediated neurotoxicity","volume":"17","author":"McIntosh LJ","year":"1996","journal-title":"Neurotoxicology"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-5-1-25"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/271676a0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(94)00001-S"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)90174-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000125210"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(84)90102-3"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-117-6-2505"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-3-10-1674"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1150459"},{"key":"e_1_2_6_27_1","first-page":"505","article-title":"Mineralocorticoid receptors mediate the enhancing effects of corticosterone on convulsion susceptibility in mice","volume":"270","author":"Roberts AJ","year":"1994","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(94)90147-3"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)69104-5"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1523\/JNEUROSCI.05-05-01228.1985","article-title":"A mechanism for glucocorticoid toxicity in the hippocampus: increased neuronal vulnerability to metabolic insults","volume":"5","author":"Sapolsky RM","year":"1985","journal-title":"J Neurosci"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000124561"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90180-1"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.4035356"},{"key":"e_1_2_6_34_1","first-page":"121","volume-title":"Neurochemistry\u2014a practical approach","author":"Seroogy KB","year":"1996"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(83)90113-6"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022010904600"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90778-A"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90207-C"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.19.120185.001233"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.1076","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.1076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T11:23:25Z","timestamp":1696937005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.1076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,18]]},"references-count":38,"aliases":["10.1002\/jnr.1076.abs"],"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,5]]}},"alternative-id":["10.1002\/jnr.1076"],"URL":"https:\/\/doi.org\/10.1002\/jnr.1076","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,18]]}}}