{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T11:36:00Z","timestamp":1771068960762,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,2,25]],"date-time":"2004-02-25T00:00:00Z","timestamp":1077667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2004,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have revealed that neural precursor cells can be expanded not only from the subventricular zone and hippocampus but also from other regions of the human embryonic brain. To determine the regional differences of these precursor cells, we divided the brain of a 9\u2010week\u2010old human embryo into four parts, i.e., telencephalon, diencephalon, mesencephalon, and rhombencephalon. All cultures of the tissues yielded neurospheres, and these spheres gave rise to neurons, astrocytes, and oligodendrocytes. An analysis of clonal populations revealed that these precursor cells were multipotent, and two region\u2010specific differences in neural precursor cells were revealed: 1) The precursor cells from the rostral part of the brain tended to proliferate faster than those from the caudal part, and 2) the precursor cells from the diencephalon and mesencephalon gave rise to more tyrosine hydoxylase (TH)\u2010positive neurons than those from the telencephalon and rhombencephalon. When 50\u2010day\u2010cultured spheres were caused to differentiate, the percentage of TH\u2010positive cells per total cell population was 1.2% for diencephalic and mesencephalic precursors, whereas it was 0.4% for telencephalic and rhombencephalic ones. Furthermore, the TH\u2010positive cells from diencephalic and mesencephalic precursors were large, multipolar, and \u03b3\u2010aminobutyric acid (GABA)\u2010negative, which suggested that these cells were midbrain dopaminergic neurons. In contrast, TH\u2010positive cells from telencephalic and rhombencephalic precursors were small, bipolar, and GABA\u2010positive. These results suggest that human neural precursor cells might have the potential to differentiate into a variety of cells but retain regional specificity. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20046","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T20:18:17Z","timestamp":1077826697000},"page":"817-824","source":"Crossref","is-referenced-by-count":50,"title":["Neural precursor cells derived from human embryonic brain retain regional 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