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Previous studies have shown that the severity of MS is reduced during pregnancy, suggesting that the increased level of sex hormones may reduce the autoimmune response. Recently, we have shown that estrogen treatment confers protection from experimental autoimmune encephalomyelitis (EAE), which is an animal model for MS. However, the cellular basis of estrogen's action remains unknown. In the current study, we demonstrate that estrogen treatment led to the induction of a novel subpopulation of regulatory cells in spleen and CNS, which also occurs naturally in pregnant mice. These previously uncharacterized cells display a low level expression of CD45 (CD45<jats:sup>dim<\/jats:sup>) and no detectable expression of many cell surface markers related to TCR signaling, including CD3 and TCR. However, these cells retained expression of VLA\u20104, an extracellular protein involved in cellular migration. Several lines of evidence suggest that these novel cells, defined as CD45<jats:sup>dim<\/jats:sup>VLA\u20104<jats:sup>+<\/jats:sup> cells, may play a role in the protective effects of estrogen in EAE. Injection of purified CD45<jats:sup>dim<\/jats:sup>VLA\u20104<jats:sup>+<\/jats:sup> cells conferred protection from spontaneous EAE (Sp\u2010EAE). In contrast, injection of CD45<jats:sup>high<\/jats:sup>VLA\u20104<jats:sup>+<\/jats:sup> cells exacerbated the disease course. CD45<jats:sup>dim<\/jats:sup>VLA\u20104<jats:sup>+<\/jats:sup> cells also suppressed antigen\u2010specific proliferation of primed lymphocytes in coculture. A better understanding of how CD45<jats:sup>dim<\/jats:sup>VLA\u20104<jats:sup>+<\/jats:sup> cells suppress the harmful immune response of EAE may help in explaining the induction of immune tolerance during pregnancy and lead to novel therapeutic approaches to combat MS and other autoimmune diseases. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20145","type":"journal-article","created":{"date-parts":[[2004,5,5]],"date-time":"2004-05-05T22:06:54Z","timestamp":1083794814000},"page":"119-126","source":"Crossref","is-referenced-by-count":35,"title":["Estrogen treatment induces a novel population of regulatory cells, which suppresses experimental autoimmune encephalomyelitis"],"prefix":"10.1002","volume":"77","author":[{"given":"Agata","family":"Matejuk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony 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