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Schwann cells are decisive in recovery of peripheral nerves after injury. In a clinical context, the use of enriched adult Schwann cells is necessary for autologous cell transplantation within nerve transplants for peripheral nerve repair. Different parameters such as tissue preparation, culture conditions, and protocols for enrichment, elevation of proliferation rates, and transfection were evaluated in cell cultures harvested from adult rat peripheral nerves. Cell preparation from in vivo predegenerated adult rat sciatic nerves combined with the use of melanocyte growth medium supplemented with forskolin, fibroblast growth factor (FGF)\u20102, and pituitary extract as a selective, serum\u2010free culture medium, with a secondary cell\u2010enrichment step using specific detachment, resulted in highly enriched cultures of adult rat Schwann cells (&gt;90%) with enhanced proliferation rates (\u226540%). About 20% of these adult Schwann cells could be modified genetically using an optimized electroporation protocol. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20166","type":"journal-article","created":{"date-parts":[[2004,5,20]],"date-time":"2004-05-20T22:05:54Z","timestamp":1085090754000},"page":"453-461","source":"Crossref","is-referenced-by-count":58,"title":["Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann 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