{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:03:58Z","timestamp":1789949038970,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,7,30]],"date-time":"2004-07-30T00:00:00Z","timestamp":1091145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2004,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transplantation of human neural stem cells (NSCs) is a promising potential therapy for neurologic dysfunctions after the hyperacute stage of stroke in humans, but large amounts of human NSCs must be expanded in long\u2010term culture for such therapy. To determine their possible therapeutic potential for human stroke, human fetal neural stem\/progenitor cells (NSPCs) (i.e., neurosphere\u2010forming cells) were isolated originally from forebrain tissues of one human fetus, and expanded in long\u2010term neurosphere culture (exceeding 24 weeks), then xenografted into the lesioned areas in the brains of Mongolian gerbils 4 days after focal ischemia. Sensorimotor and cognitive functions were evaluated during the 4 weeks after transplantation. The total infarction volume in the NSPC\u2010grafted animals was significantly lower than that in controls. Approximately 8% of the grafted NSPCs survived, mainly in areas of selective neuronal death, and were costained with antibodies against neuronal nuclei antibody (NeuN), microtubule associated protein (MAP\u20102), glial fibrillary acidic protein (GFAP), and anti\u20102\u20323\u2032 cyclic nucleotide 3\u2032\u2010phosphodiesterase (CNPase). Synaptic structures between NSPCs\u2010derived neurons and host neurons were observed. Furthermore, gradual improvement of neurologic functions was observed clearly in the NSPC\u2010grafted animals, compared to that in controls. Human NSPCs, even from long\u2010term culture, remarkably improved neurologic functions after focal ischemia in the Mongolian gerbil, and maintained their abilities to migrate around the infarction, differentiate into mature neurons, and form synapses with host neuronal circuits. These results indicate that in vitro\u2010expanded human neurosphere cells are a potential source for transplantable material for treatment of stroke. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20246","type":"journal-article","created":{"date-parts":[[2004,8,2]],"date-time":"2004-08-02T22:01:22Z","timestamp":1091484082000},"page":"215-223","source":"Crossref","is-referenced-by-count":151,"title":["Human neural stem\/progenitor cells, expanded in long\u2010term neurosphere culture, promote functional recovery after focal ischemia in Mongolian 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