{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:38:17Z","timestamp":1790789897384,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2004,11,22]],"date-time":"2004-11-22T00:00:00Z","timestamp":1101081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Inflammatory\u2010activated glia are seen in numerous central nervous system (CNS) pathologies and can kill nearby neurons through the release of cytotoxic mediators. Glia, when activated, can express the inducible isoform of nitric oxide synthase (iNOS) producing high levels of nitric oxide (NO), which can kill neurons in certain conditions. We show, however, that inflammatory activation of glia in a mature culture of cerebellar granule neurons and glia causes little or no neuronal death under normal (21%) oxygen conditions. Similarly, hypoxia (2% oxygen) or low levels of an NO donor (100 \u03bcM DETA\/NO) caused little or no neuronal death in nonactivated cultures. If inflammatory activation of glia or addition of NO donor was combined with hypoxia, however, extensive neuronal death occurred. Death in both cases was prevented by the\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyl\u2010\n                    <jats:sc>D<\/jats:sc>\n                    \u2010aspartate (NMDA) receptor blocker MK\u2010801, implying that death was mediated by the glutamate receptor. Low levels of NO were found to increase the apparent K\n                    <jats:sub>M<\/jats:sub>\n                    of cellular oxygen consumption for oxygen, probably due to NO\u2010induced inhibition of mitochondrial respiration, in competition with oxygen, at cytochrome oxidase. Necrotic death, induced by hypoxia plus DETA\/NO, was increased further by deoxyglucose, an inhibitor of glycolysis, suggesting that necrosis was mediated by energy depletion. Hypoxia was found to be a potent stimulator of microglia proliferation, but this proliferation was not significant in inflammatory\u2010activated cultures. These results suggest that low levels of NO can induce neuronal death under hypoxic conditions, mediated by glutamate after NO inhibition of respiration in competition with oxygen. Brain inflammation can thus sensitize to hypoxia\u2010induced death, which may be important in pathologies such as stroke, neurodegeneration, and brain aging. \u00a9 2004 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jnr.20285","type":"journal-article","created":{"date-parts":[[2004,11,22]],"date-time":"2004-11-22T18:21:50Z","timestamp":1101147710000},"page":"208-215","source":"Crossref","is-referenced-by-count":95,"title":["Nitric oxide from inflammatory\u2010activated glia synergizes with hypoxia to induce neuronal death"],"prefix":"10.1002","volume":"79","author":[{"given":"Palwinder","family":"Mander","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilma","family":"Borutaite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Moncada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy 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