{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:00:49Z","timestamp":1774321249744,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2004,11,30]],"date-time":"2004-11-30T00:00:00Z","timestamp":1101772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Region\u2010selective accumulation of brain lactate occurs in TD; however, the mechanisms responsible have not been elucidated fully. <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C nuclear magnetic resonance (NMR) spectroscopy were therefore used to investigate de novo lactate synthesis from [1\u2010<jats:sup>13<\/jats:sup>C]glucose in vulnerable (medial thalamus) and nonvulnerable (frontal cortex) brain regions of rats made thiamine deficient by administration of the central thiamine antagonist pyrithiamine. De novo synthesis of lactate was increased in the medial thalamus to 148% and 226% of pair\u2010fed control values at presymptomatic and symptomatic stages of thiamine deficiency, respectively, whereas no such changes were observed in the frontal cortex. Administration of a glucose load selectively worsened the changes in medial thalamus. Pyruvate recycling and peripherally derived lactate did not contribute significantly to the lactate increase within the thiamine\u2010deficient brain. Increases in immunolabeling of the lactate dehydrogenase isoenzymes (LDH1 and LDH5) were observed in the medial thalamus of thiamine\u2010deficient animals. Metabolic impairment due to thiamine deficiency thus results in increased glycolysis, increased LDH immunolabeling of neurons and astrocytes and increased de novo synthesis of lactate in brain regions vulnerable to thiamine deficiency. These results are consistent with the notion that focal lactate accumulation participates in the worsening of neurologic symptoms in thiamine\u2010deficient patients. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20290","type":"journal-article","created":{"date-parts":[[2004,11,30]],"date-time":"2004-11-30T18:46:43Z","timestamp":1101840403000},"page":"33-41","source":"Crossref","is-referenced-by-count":30,"title":["Brain lactate synthesis in thiamine deficiency: A re\u2010evaluation using <sup>1<\/sup>H\u2010<sup>13<\/sup>C nuclear magnetic resonance spectroscopy"],"prefix":"10.1002","volume":"79","author":[{"given":"Darren","family":"Navarro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Zwingmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan S.","family":"Hazell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger F.","family":"Butterworth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0220988"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.65052178.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb01850.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.136.3520.962"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38247-X"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1983.73"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410160609"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-5994.1980.tb03720.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.65052227.x"},{"key":"e_1_2_5_11_1","first-page":"95","article-title":"Animal models of the Wernicke\u2010Korsakoff Syndrome","volume":"22","author":"H\u00e9roux M","year":"1992","journal-title":"Neuromethods"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00995157"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1968.tb08961.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.20.6426"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00203-0"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2004.19.1.42"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0240711"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.53.8.697"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1982.tb31210.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105912"},{"key":"e_1_2_5_21_1","first-page":"331","article-title":"Wernicke's encephalopathy in a child with acute lymphoblastic leukemia","volume":"15","author":"Miyajima Y","year":"1993","journal-title":"Am J Pediatr Hematol Oncol"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(93)90107-A"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(96)06385-1"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.142.3595.1075"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20001015)62:2<286::AID-JNR13>3.0.CO;2-0"},{"key":"e_1_2_5_26_1","first-page":"537","article-title":"Studies on the physiological functions of thiamine. 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