{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:08:16Z","timestamp":1790680096593,"version":"4.1.0"},"reference-count":75,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,28]],"date-time":"2005-03-28T00:00:00Z","timestamp":1111968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neurogenesis in the olfactory epithelium (OE) is induced by olfactory bulbectomy (OBX), which effectively axotomizes olfactory sensory neurons (OSNs) and removes their synaptic targets, resulting in apoptosis. We used Affymetrix high\u2010density oligonucleotide arrays to investigate changes in gene expression during initiation of signaling in pathways that regulate apoptosis and neurogenesis in the murine OE at 2, 8, 16, and 48 hr after bilateral OBX compared to that in sham\u2010operated controls. We focused on regulation of a defined set of genes associated with apoptosis, stem\/progenitor cell regulation, and cell cycle progression because of the activation of these processes in OE degeneration and remodeling after OBX. After data scrubbing and categorical analysis, one\u2010way analysis of variance identified 72 genes (4.9% of the present known genes) as being regulated significantly (<jats:italic>P<\/jats:italic> &lt; 0.05) at one or more points; 50 were defined as regulated differentially with the false discovery rate at 10%. Significant changes in gene expression occurred in all categories as early as 2 hr post\u2010OBX, with the greatest number of differentially regulated genes at 16 and 48 hr. Hierarchical cluster analysis and correlation coefficients were used to identify similarities in patterns of gene expression changes within and across categories. Validation was carried out with SuperArray macroarrays and real\u2010time RT\u2010PCR. Our results confirmed the participation of many genes in known signaling pathways and identified changes in the expression of 42 genes not identified previously as participating in apoptosis and neurogenesis in the OE. Additionally, our analyses indicated the early involvement of genes regulating cytoskeletal reorganization and angiogenesis in the response to OBX. These studies are an important first step in defining early time\u2010dependent changes in gene expression after target ablation that lead to neurogenesis in the olfactory sensory epithelium. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20411","type":"journal-article","created":{"date-parts":[[2005,3,28]],"date-time":"2005-03-28T19:47:05Z","timestamp":1112039225000},"page":"309-329","source":"Crossref","is-referenced-by-count":25,"title":["Temporal profiling of gene expression during neurogenesis and remodeling in the olfactory epithelium at short intervals after target ablation"],"prefix":"10.1002","volume":"80","author":[{"given":"Thomas V.","family":"Getchell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhika A.","family":"Vaishnav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Kwong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold J.","family":"Stromberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn L.","family":"Getchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,3,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10160"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9532"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10147"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199605)30:1<67::AID-NEU7>3.0.CO;2-E"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02040"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0330-04.2004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10148"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.8.1871"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.11.2323"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10150"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-18-07099.2001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1993.1480"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.139.3.1147"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2003.08.012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00533.2002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200212060"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2001.01327.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00462"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg758"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00120.2002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00117.2003"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-1637(02)00066-1"},{"key":"e_1_2_6_25_1","first-page":"185","article-title":"Horizontal basal cell proliferation in the olfactory epithelium of transforming growth factor\u2010\u03b1 transgenic mice","volume":"299","author":"Getchell TV","year":"2000","journal-title":"Cell Tissue Res"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20157"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10090"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10432"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2003.09.024"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.1191"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00770-0"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1096\/fasebj.9.10.7542216","article-title":"Expression and inducibility of vascular adhesion receptors in development","volume":"9","author":"Heyward SA","year":"1995","journal-title":"FASEB J"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903630111"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2001.01336.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0402-e101"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0025com"},{"key":"e_1_2_6_37_1","first-page":"2152","article-title":"Mechanisms of mitotic inhibition in corneal endothelium: contact inhibition and TGF\u2010\u03b22","volume":"43","author":"Joyce NC","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19981116)401:2<205::AID-CNE4>3.0.CO;2-4"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00187.2004"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.10275"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020222600711"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00009-5"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(96)00068-5"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000081429.89277.AF"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19991001)58:1<152::AID-JNR15>3.0.CO;2-V"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199406270-00008"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2003.08.002"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.20.11167"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1193"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318813"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20049"},{"key":"e_1_2_6_52_1","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1242\/dev.129.11.2649","article-title":"Directed differentiation of pluripotent cells to neural lineages: homogeneous formation and differentiation of a neuroectoderm population","volume":"129","author":"Rathjen J","year":"2002","journal-title":"Development"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btf877"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199708)209:4<418::AID-AJA9>3.0.CO;2-L"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0901-823"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299909034666"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.1132"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.6.1403"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-11-03556.1991"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0402-e65"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.10047"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903590103"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095505"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1995.1099"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318179"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.13.5919"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00229-0"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(98)00016-4"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092436"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2003.1110124.x"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5457.1427"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-1878(200010)22:10<911::AID-BIES6>3.0.CO;2-V"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01172-8"},{"key":"e_1_2_6_74_1","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1242\/dev.127.11.2367","article-title":"Sox2 regulatory sequences direct expression of a \u03b2\u2010geo transgene to telencephalic neural stem cells and precursors of the mouse embryo, revealing regionalization of gene expression in CNS stem cells","volume":"127","author":"Zappone MV","year":"2000","journal-title":"Development"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02041"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405350101"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.20411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.20411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T20:04:22Z","timestamp":1700165062000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.20411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,28]]},"references-count":75,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,5]]}},"alternative-id":["10.1002\/jnr.20411"],"URL":"https:\/\/doi.org\/10.1002\/jnr.20411","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,28]]}}}