{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:05:01Z","timestamp":1792724701581,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T00:00:00Z","timestamp":1151280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gene transfer is a powerful tool for functional gene analysis in human cells. In this respect, there is a need to develop experimental models that involve homogeneous cultures of human neuron\u2010like cells susceptible to gene transduction and that are easy to handle. Here we describe an optimized and reproducible procedure to differentiate human SH\u2010SY5Y neuroblastoma cells into a homogeneous population of neuron\u2010like cells. The fully differentiated cells are postmitotic and resemble primary cultured neurons in terms of their cytoskeletal polarity. Notably, differentiated SH\u2010SY5Y cells are far more susceptible to transduction by herpes simplex virus (HSV\u20101)\u2010based vectors than proliferating SH\u2010SY5Y cells. This increase in transduction efficiency after neuronal differentiation may be due to the up\u2010regulation of cell surface receptors for herpesvirus entry. In summary, we propose that fully differentiated human neuron\u2010like cells obtained from the SH\u2010SY5Y neuroblastoma may constitute an excellent and versatile experimental tool for gene transfer and functional genomic studies with HSV\u20101 vectors. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.20976","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T23:05:55Z","timestamp":1151363155000},"page":"755-767","source":"Crossref","is-referenced-by-count":48,"title":["Differentiation of a human neuroblastoma into neuron\u2010like cells increases their susceptibility to transduction by herpesviral vectors"],"prefix":"10.1002","volume":"84","author":[{"given":"Alfredo","family":"Gimenez\u2010Cassina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Diaz\u2010Nido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/10430340252837251"},{"key":"e_1_2_6_3_1","first-page":"147","article-title":"Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera\u20102. 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