{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T12:43:18Z","timestamp":1776861798771,"version":"3.51.2"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,5,10]],"date-time":"2007-05-10T00:00:00Z","timestamp":1178755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Embryonic stem cell (ESC)\u2013derived products have emerged as a promising cell source for neuroregeneration. C17.2 neural precursor cells were noted to express genes of neurotrophins and neuroprotective factors and to be enable to enhance proliferation, neuritogenesis, and differentiation of SH\u2010SY5Y and SK\u2010N\u2010AS neuroblasts, suggesting their neurotrophic potential. We used C17.2 cells as neurotrophic chaperones to induce ESCs, D3, and E14TG2a into neural lineage cells. Significantly greater numbers of Sox\u20102<jats:sup>+<\/jats:sup>, Musashi\u20101<jats:sup>+<\/jats:sup>, and nestin<jats:sup>+<\/jats:sup> neurospheres developed in noncontact cocultures than in cultures of ESCs without C17.2 support or with 50% conditioned medium after 8 days. Immunoreactivity of the neuronal, astrocytic and oligodendrocytic markers was evident in cultures further differentiated for 10 days. Expression of <jats:italic>Pax\u20106, Otx\u20101,<\/jats:italic> and <jats:italic>Nurr\u20101<\/jats:italic> genes suggested neuroectodermal precursors in products encompassing neural stem cells, dopaminergic neurons, astrocytes, and oligodendrocytes. <jats:italic>\u03b1\u2010Fetoprotein, GATA\u20104, Brachyury, Nkx\u20102.5,<\/jats:italic> and <jats:italic>Myf\u20105<\/jats:italic> genes were not detected, indicating any mesodermal and endodermal cells. However, weak expression of <jats:italic>Oct\u20104<\/jats:italic> was noted. Behavioral assessment of ischemic mice 2 weeks after transplantation revealed significant improvement in cognitive function compared with that in ischemic sham\u2010operated mice. Tracking bromodeoxyuridine\u2010labeled products demonstrated that mostly implanted cells were localized along the needle track of the injection in the brain parenchyma, whereas some migrated to the striatum, cortex, nerve fiber bundle of the corpus callosum, and hippocampus in the ipsilateral hemisphere. One episode (of 22) of teratoma development was noted. Data from this study suggest a paradigm of trophism of neural progenitor cells for induction of ESCs into neural lineage cells. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21319","type":"journal-article","created":{"date-parts":[[2007,5,10]],"date-time":"2007-05-10T22:21:09Z","timestamp":1178835669000},"page":"1851-1862","source":"Crossref","is-referenced-by-count":23,"title":["Trophism of neural progenitor cells to embryonic stem cells: Neural induction and transplantation in a mouse ischemic stroke model"],"prefix":"10.1002","volume":"85","author":[{"given":"Shu Pan","family":"Fong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kam Sze","family":"Tsang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy Bik 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