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To determine whether MN can modulate the expression of chemokine receptors and interleukin\u201010 (IL10) in a model of neonatal brain injury, we produced an HI insult to the right cerebral hemisphere (ipsilateral) of the 7\u2010day\u2010old rat (PD7) by right common carotid artery ligation and 2.25 hr of hypoxia in 8% oxygen. MN (45 mg\/kg, i.p.) or vehicle (PBS) was injected twice: 2 days and immediately before the HI insult. At 0, 1, 3, and 24 hr and 14 days after HI, total RNA from the ipsilateral and contralateral (exposed to hypoxia only) hemispheres was extracted, reverse transcribed, and amplified with gene\u2010specific primers using a semiquantitative RT\u2010PCR for macrophage inflammatory protein\u20101\u03b1), interferon\u2010inducible protein (IP\u201010), C\u2010C chemokine receptor 5 (CCR5; MIP\u20101\u03b1 receptor), C\u2010X\u2010C chemokine receptor 3 (CXCR3; IP\u201010 receptor), and IL10. We found that, in the ipsilateral hemisphere, a significant (<jats:italic>P<\/jats:italic> &lt; 0.05) increase in MIP\u20101\u03b1, IP\u201010, CCR5, and CXCR3 mRNA levels was observed. MN treatment decreased mRNA levels for CCR5 and CXCR3. In contrast, the levels of antiinflammatory cytokine IL10 were markedly decreased as a result of HI insult. Treatment with MN, however, had no effect on IL10. We conclude that MN decreased proinflammatory chemokine receptor expression but had little or no influence on the expression of antiinflammatory cytokine IL10. These effects confirm the antiinflammatory effect of MN in neonatal HI brain injury. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21380","type":"journal-article","created":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T22:48:01Z","timestamp":1180997281000},"page":"2450-2459","source":"Crossref","is-referenced-by-count":31,"title":["Minocycline modulates chemokine receptors but not interleukin\u201010 mRNA expression in hypoxic\u2010ischemic neonatal rat brain"],"prefix":"10.1002","volume":"85","author":[{"given":"Sergey G.","family":"Kremlev","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca 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