{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:24:57Z","timestamp":1762100697054},"reference-count":40,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2007,7,12]],"date-time":"2007-07-12T00:00:00Z","timestamp":1184198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hereditary cerebral hemorrhage with amyloidosis, Dutch type (HCHWA\u2010D), is an autosomal dominant disorder caused by the Dutch mutation (E693Q) in the \u03b2\u2010amyloid precursor protein. This mutation produces an aberrant amyloid \u03b2 (A\u03b2) species (A\u03b2E22Q) and causes severe meningocortical vascular A\u03b2 deposition. We analyzed the A\u03b2 composition of the vascular amyloid in the brains of HCHWA\u2010D patients. Immunohistochemistry demonstrated that the vascular amyloid contained both A\u03b240 and A\u03b242, with a high A\u03b240\/A\u03b242 ratio. In Western blotting of cerebral microvessel fractions isolated from the brains, both wild\u2010type and Dutch\u2010type A\u03b240 were observed as major species. Reverse\u2010phase HPLC\u2010mass spectrometric analysis of the fractions revealed both wild\u2010type and Dutch\u2010type A\u03b238 as the other main components of the vascular amyloid. Moreover, we detected peaks corresponding to Dutch\u2010type A\u03b242 but not to wild\u2010type A\u03b242. These results suggest a pathogenic role for the mutant A\u03b242 in addition to the mutant A\u03b240 in the cerebral amyloid angiopathy of HCHWA\u2010D. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21413","type":"journal-article","created":{"date-parts":[[2007,7,12]],"date-time":"2007-07-12T21:22:34Z","timestamp":1184275354000},"page":"2917-2923","source":"Crossref","is-referenced-by-count":16,"title":["Cerebral vascular accumulation of Dutch\u2010type A\u03b242, but not wild\u2010type A\u03b242, in hereditary cerebral hemorrhage with amyloidosis, Dutch 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