{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:56:03Z","timestamp":1779148563403,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,11,20]],"date-time":"2007-11-20T00:00:00Z","timestamp":1195516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neonatal hypoxia\u2010ischemia (HI) induces immediate early gene (IEG) <jats:italic>c\u2010fos<\/jats:italic> expression as well as neuron death. The precise role of IEGs in neonatal HI is unclear. We investigated the temporal and spatial patterns of c\u2010Fos expression in postnatal day 7 mice after unilateral carotid ligation and exposure to 8% oxygen. mRNA levels of <jats:italic>c\u2010fos<\/jats:italic> quantitated by real\u2010time polymerase chain reaction (PCR) increased nearly 40\u2010fold (log 1.2 \u00b1 0.4) in the ipsilateral hippocampus 3 hr following neonatal HI, then returned to basal levels within 12 hr, although no change was observed in <jats:italic>c\u2010jun<\/jats:italic> mRNA. Frozen coronal brain sections were stained with cresyl violet or used for immunohistochemical detection of c\u2010Fos, cleaved caspase\u20103, glial fibrillary acidic protein (GFAP), and the mature neuron marker NeuN. c\u2010Fos immunoreactivity increased throughout the injured hippocampus 3 hr after HI but became restricted to the CA2\u20133 subregion and the dentate gyrus (DG) at 6\u201312 hr and declined by 24 hr. In contrast, cleaved (activated) caspase\u20103 immunoreactivity was most abundant in the ipsilateral CA1 region at 3\u20136 hr after neonatal HI, then became more prominent in CA2\u20133 and DG. Double\u2010labeling experiments showed c\u2010Fos and cleaved caspase\u20103 immunoreactivity localized in spatially distinct neuron subpopulations. Prominent c\u2010Fos immunoreactivity was observed in surviving CA2\u20133 and external granular DG neurons, and robust cleaved caspase\u20103 immunoreactivity was observed in pyknotic CA1, CA2\u20133, and subgranular DG neurons. The differential expression of c\u2010Fos in HI\u2010resistant hippocampal subpopulations vs. cleaved caspase\u20103 in dying neurons suggests a neuroprotective role for c\u2010Fos expression in neonatal HI. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21573","type":"journal-article","created":{"date-parts":[[2007,11,20]],"date-time":"2007-11-20T18:16:52Z","timestamp":1195582612000},"page":"1115-1124","source":"Crossref","is-referenced-by-count":24,"title":["Differential activation of c\u2010fos and caspase\u20103 in hippocampal neuron subpopulations following neonatal hypoxia\u2010ischemia"],"prefix":"10.1002","volume":"86","author":[{"given":"Jayne M.","family":"Ness","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cary R.","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason D.","family":"Washington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin A.","family":"Roth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven L.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90495-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)02622-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.27.9.1682"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0736-5748(94)90012-4"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1992.136"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(97)00139-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2003.12.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200105000-00009"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90274-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1209"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01201-2"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041996"},{"key":"e_1_2_6_14_1","volume-title":"The mouse brain in stereotaxic coordinates","author":"Franklin KBJ","year":"1997"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401871"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(93)90194-T"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90763-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204387"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.09.020"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80068-3"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(03)00005-4"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199709080-00024"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.109.1.116"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(98)00039-4"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(94)90218-6"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.04.132"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2005.07.003"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.21.1.107"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1992.57"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7248"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2006.03.006"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.12b.1687"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0502-e131"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00892-0"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/44.11.8918908"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90105-M"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400449"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050951"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(98)00077-7"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90665-I"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19980801)53:3<330::AID-JNR7>3.0.CO;2-B"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000047087"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng859"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3795-06.2006"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01459-6"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401545"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.21573","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.21573","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T12:14:51Z","timestamp":1696940091000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.21573"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11,20]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["10.1002\/jnr.21573"],"URL":"https:\/\/doi.org\/10.1002\/jnr.21573","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,11,20]]}}}