{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T10:41:51Z","timestamp":1764499311955},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,1,8]],"date-time":"2008-01-08T00:00:00Z","timestamp":1199750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2008,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hippocalcin is a Ca<jats:sup>2+<\/jats:sup>\u2010binding protein that is expressed mainly in pyramidal nerve cells of the hippocampus. However, its functions and mechanism in the brain remain unclear. To elucidate the role of hippocalcin, we used a conditionally immortalized hippocampal cell line (H19\u20107) and showed that bFGF treatment increased the expression of hippocalcin during bFGF\u2010induced neurite outgrowth of H19\u20107 cells. Overexpression of hippocalcin dramatically elongated neurites and increased the expression of basic helix\u2013loop\u2013helix transcription factor, that is, NeuroD without bFGF stimulation. Treatment of the cells with hippocalcin siRNA completely blocked bFGF\u2010induced neurite outgrowth and NeuroD expression. bFGF stimulation resulted in activation of phospholipase C\u2013\u03b3 (PLC\u2010\u03b3) and an increased level of intracellular Ca<jats:sup>2+<\/jats:sup>. Hippocalcin expression by bFGF stimulation was fully blocked by both the PLC\u2010\u03b3 inhibitor U73122 and BAPTA\u2010AM, a chelator of intracellular Ca<jats:sup>2+<\/jats:sup>, suggesting that hippocalcin expression by bFGF is dependent on PLC\u2010\u03b3 and Ca<jats:sup>2+<\/jats:sup>. Moreover, both U73122 and BAPTA\u2010AM completely blocked bFGF\u2010induced neurite outgrowth and NeuroD expression. Taken together, these results suggest for the first time that bFGF induces hippocalcin expression in H19\u20107 cells through PLC\u2010\u03b3 activation, which leads to neurite outgrowth. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21602","type":"journal-article","created":{"date-parts":[[2008,1,8]],"date-time":"2008-01-08T23:46:42Z","timestamp":1199836002000},"page":"1557-1565","source":"Crossref","is-referenced-by-count":14,"title":["A novel role of hippocalcin in bFGF\u2010induced neurite outgrowth of H19\u20107 cells"],"prefix":"10.1002","volume":"86","author":[{"given":"Doo\u2010Yi","family":"Oh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju Hwan","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Young","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong Seok","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010Ju","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin Hee","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang Chul","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki Sung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joong\u2010Soo","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00230.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.4.2272"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90409-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4213"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.10.4373"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021000425070"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1139\/o97-091"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.7.3966"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00544-4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100590000028"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90461-7"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.02.034"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46711-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.08.015"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7754368"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200004)217:4<361::AID-DVDY3>3.0.CO;2-8"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-9639(02)00454-5"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90091-8"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.14.3597"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111750200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200306042"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20665"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312766200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.06.014"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3602"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90654-4"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(94)90105-8"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90112-4"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(99)80052-X"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5394.1705"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90068-3"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1242\/dev.120.6.1685","article-title":"Characterisation of the second messenger pathway underlying neurite outgrowth stimulated by FGF","volume":"120","author":"Williams EJ","year":"1994","journal-title":"Development"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb895"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.21602","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.21602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:18:12Z","timestamp":1697156292000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.21602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1,8]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2008,5,15]]}},"alternative-id":["10.1002\/jnr.21602"],"URL":"https:\/\/doi.org\/10.1002\/jnr.21602","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1,8]]}}}