{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:05:09Z","timestamp":1762160709161},"reference-count":66,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,4,25]],"date-time":"2008-04-25T00:00:00Z","timestamp":1209081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2008,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A growth factor may have different actions depending on developmental stage. We investigated this phenomenon in the interactions of fibroblast growth factor 2 (FGF2) and neurotrophins on cochlear ganglion (CG) development. The portions of the otocyst fated to form the CG and cochlear epithelium were cocultured at embryonic day 11 (E11). Cultures were divided into groups fed with defined medium, with or without FGF2 and neurotrophin supplements, alone or in combination, for 7 days. We measured the number of migrating neuroblasts and distances migrated, neurite outgrowth, and axonlike processes. We used immunohistochemistry to locate neurotrophin 3 (NT3) and its high\u2010affinity receptor (TrkC) in the auditory system, along with FGF2 and its R1 receptor, at comparable developmental stages in vitro and in situ from E11 until birth (P1) in the precursors of hair cells, support cells, and CG cells. Potential sites for interaction were localized to the nucleus, perikaryal cytoplasm, and cell surfaces, including processes and growth cones. Time\u2010lapse imaging and quantitative measures support the hypothesis that FGF2 alone or combined with neurotrophins promotes migration and neurite outgrowth. Synergism or antagonism between NT3 and other factors suggest interactions at the receptor level. Formation of axons, endings, and synaptic vesicle protein 2 were increased by interactions of NT3 and FGF2. Similar experiments with a mutant overexpressor for FGF2 suggest that endogenous FGF2 supports migration and neurite outgrowth of CG neuroblasts as well as proliferation, leading to accelerated development. The findings suggest interactive and sequential roles for FGF2 and NT3. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21685","type":"journal-article","created":{"date-parts":[[2008,4,26]],"date-time":"2008-04-26T00:17:01Z","timestamp":1209169021000},"page":"2376-2391","source":"Crossref","is-referenced-by-count":25,"title":["Interactive roles of fibroblast growth factor 2 and neurotrophin 3 in the sequence of migration, process outgrowth, and axonal differentiation of mouse cochlear ganglion cells"],"prefix":"10.1002","volume":"86","author":[{"given":"Waheeda A.","family":"Hossain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chrystal","family":"D'Sa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Kent","family":"Morest","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00378"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90053-3"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2005.06.013"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/7.4.293"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.6.12.1861"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199810)177:1<123::AID-JCP13>3.0.CO;2-E"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199111000-00001"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4213"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05695.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.2.491"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490270103"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1992.tb00141.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90263-5"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"6170","DOI":"10.1523\/JNEUROSCI.21-16-06170.2001","article-title":"Spatial shaping of cochlear innervation by temporally regulated neurotrophin expression","volume":"21","author":"Fari\u00f1as I","year":"2001","journal-title":"J Neurosci"},{"key":"e_1_2_5_16_1","first-page":"401","article-title":"Reduction and loss of inner ear innervation in trkB and trkC receptor knockout mice: a whole mount DiI and scanning electron microscopic analysis","volume":"1","author":"Fritzsch B","year":"1995","journal-title":"Aud Neurosci"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-16-06213.1997"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(96)01007-7"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903410304"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90412-X"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)47102-4"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(86)90001-8"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-5748(99)00043-X"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960408)367:3<454::AID-CNE10>3.0.CO;2-H"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199606)30:2<246::AID-NEU6>3.0.CO;2-5"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-06-01959.1997"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20001001)62:1<40::AID-JNR5>3.0.CO;2-L"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1996.0052"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6623"},{"key":"e_1_2_5_30_1","first-page":"21329","article-title":"Expression of fibroblast growth factor receptor FGFR 1 and 2 during early development of mouse cochlear nucleus and ganglion","author":"Hossain WA","year":"2001","journal-title":"Assoc Res Otolaryngol Abs"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2002.7872"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20264"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00068-9"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.5.1661"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1273605"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199404000-00003"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.19.030196.001445"},{"key":"e_1_2_5_38_1","first-page":"273","article-title":"In vitro study of fate mapping of the mouse otocyst","volume":"82","author":"Li CW","year":"1976","journal-title":"Trans Sect Otolaryngol Am Acad Ophthalmol Otolaryngol"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051570302"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1159\/000017361"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh706"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199606)167:3<443::AID-JCP8>3.0.CO;2-P"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(93)90106-B"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(95)00011-2"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(99)00152-5"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)91427-G"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8066451"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971006)386:4<529::AID-CNE1>3.0.CO;2-4"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(92)90178-S"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199812210-00016"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.20.9915"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(94)90064-7"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9269"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00824-3"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(87)90003-0"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1238"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903540305"},{"key":"e_1_2_5_58_1","first-page":"221","article-title":"Development of the inner ear of the mouse: a radioautographic study of terminal mitoses","volume":"220","author":"Ruben RJ","year":"1967","journal-title":"Acta Otolaryngol:Suppl"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(94)90286-0"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1139\/o99-016"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21227"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90030-6"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816x.2001.01690.x"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90124-A"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(94)90281-X"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(93)90202-C"},{"key":"e_1_2_5_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10718"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.21685","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.21685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T22:21:36Z","timestamp":1700000496000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.21685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,25]]},"references-count":66,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,8,15]]}},"alternative-id":["10.1002\/jnr.21685"],"URL":"https:\/\/doi.org\/10.1002\/jnr.21685","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,25]]}}}