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Because only 11 transcripts were significantly changed, the statistically subsequent 25 transcripts below the significance level were additionally included in a first round of qRT\u2010PCR evaluations. Furthermore, all 36 genes were then tested for mutual interactions or interactions with other molecules in a physiological context using PathwayArchitect software. Thirty of them were displayed in a network interacting with at least one partner molecule from the list or with other partner molecules known from the literature. All partner molecules from the most prominent 10 molecules of this network were then identified and put together into a new list. On those grounds, the hypothesis was made that metabolic network components of the insulin signaling pathway are perturbed in the disease. This pathway was subsequently tested by a second round of qRT\u2010PCR, adding also a few additional candidate molecules belonging to this pathway. It turned out that the key target\u2014FABP7\u2014fell into the group of transcripts not significantly regulated within the chip data, and another key target\u2014IRS1\u2014did not show up in the chip experiments at all. In conclusion, our data reveal a problem with adhering to statistical significances in microarray experiments, insofar as molecules important for the disease may fall into the range of statistical noise. This approach may also be useful to find new targets for pharmacotherapy in affective disorders. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21753","type":"journal-article","created":{"date-parts":[[2008,6,9]],"date-time":"2008-06-09T23:48:01Z","timestamp":1213055281000},"page":"3184-3193","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptional changes in insulin\u2010 and lipid metabolism\u2010related genes in the hippocampus of olfactory bulbectomized mice"],"prefix":"10.1002","volume":"86","author":[{"given":"Peter","family":"Gass","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Leonardi\u2010Essmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maha","family":"Zueger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainer","family":"Spanagel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Gebicke\u2010Haerter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/gps.666"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1302105"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2512-05.2005"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI25444"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.63051616.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M300086-JLR200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.036863"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902080208"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.167.8.802"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03194597"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1825"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-4571-z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpsyc.1997.01830190015002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.27.12.2856"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0031-6997(24)01424-8","article-title":"Enigma of the peripheral benzodiazepine receptor","volume":"51","author":"Gavish M","year":"1999","journal-title":"Pharmacol Rev"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.01.020"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-2772(03)00087-2"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00654"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2006.07.017"},{"key":"e_1_2_7_22_1","unstructured":"IrizarryRA GautierL BolstadBM CrispinM et al.2005.Affy: methods for Affymetrix oligonucleotide arrays. 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