{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T22:12:19Z","timestamp":1768255939952,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2008,7,14]],"date-time":"2008-07-14T00:00:00Z","timestamp":1215993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mechanisms underlying axon degeneration in peripheral neuropathies and during normal remodeling are poorly understood. Because estrogen induces widespread sympathetic axon degeneration in female reproductive tract smooth muscle, we surveyed estrogen\u2010regulated genes in rat myometrium. Microarray analysis revealed that the neural cell adhesion protein neurotrimin (Ntm) was markedly up\u2010regulated 6 hr and down\u2010regulated 24 hr after injection of 17\u03b2\u2010estradiol, and real time RT\u2010PCR confirmed this pattern of expression. Protein analysis by Western blotting showed that uterine Ntm protein is also up\u2010regulated in vivo 6\u201324 hr following estrogen injection and that Ntm protein is increased selectively in the myometrium during the high\u2010estrogen phase of the estrous cycle. Cultured myometrial smooth muscle cells display perinuclear accumulations of Ntm protein, and 17\u03b2\u2010estradiol also increases intracellular levels of Ntm and its secretion into the culture medium. To determine if neurotrimin is required for estrogen\u2010induced sympathetic pruning, sympathetic neurons were cocultured with uterine smooth muscle cells transfected with siRNA directed against Ntm. Although estrogen inhibited neurite outgrowth in nontransfected cocultures, estrogen's ability to reduce sympathetic outgrowth was impaired substantially following Ntm down\u2010regulation. This supports a role for neurotrimin in mediating estrogen\u2010induced sympathetic pruning in some peripheral targets. Together with earlier studies, these findings support the idea that physiological sympathetic axon degeneration is a multifactorial process requiring dynamic regulation of multiple repellant proteins. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21768","type":"journal-article","created":{"date-parts":[[2008,7,14]],"date-time":"2008-07-14T22:10:28Z","timestamp":1216073428000},"page":"3086-3095","source":"Crossref","is-referenced-by-count":36,"title":["Neurotrimin is an estrogen\u2010regulated determinant of peripheral sympathetic innervation"],"prefix":"10.1002","volume":"86","author":[{"given":"Dora","family":"Krizsan\u2010Agbas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetyana","family":"Pedchenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter 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