{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T15:06:39Z","timestamp":1697295999797},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,8,27]],"date-time":"2008-08-27T00:00:00Z","timestamp":1219795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ventilatory response to hypoxia is mediated by peripheral inputs arising from the arterial chemoreceptors. In their absence, hypoxic adaptation can be achieved, possibly as a result of central cellular reorganization. To study this reorganization, we used chemodenervated rats to investigate the expression and localization of vascular endothelial growth factor (VEGF) in the brainstem. VEGF is a target gene of hypoxia\u2010inducible factor (HIF) that is responsible for the morphofunctional remodeling induced by hypoxia. Intact and chemodenervated rats were subjected to normoxia or hypoxia for 6 hr (10% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>). VEGF protein was quantified in micropunches of brainstem tissue. Only chemodenervated animals showed an increased VEGF expression in response to hypoxia, whereas, in normoxia, VEGF expression was not modified by chemodenervation. The same hypoxic condition was repeated for 8 days before immunocytochemical staining with anti\u2010VEGF; antiglial fibrillary acidic protein (GFAP), a marker of astrocytes; and anti\u2010rat endothelial cell antigen\u20101 (anti\u2010RECA\u20101) that recognizes endothelial cells. Confocal analysis showed a cellular colocalization of GFAP and VEGF, indicating that VEGF was overexpressed predominantly in astrocytes. Increased RECA\u20101 immunolabeling indicated an enhanced angiogenesis in chemodenervated rats subjected to hypoxia. These results indicate that glial cells and the vascular network contribute to the brainstem remodeling. The peripheral chemodenervation reveals a central O<jats:sub>2<\/jats:sub> chemosensitivity involving a cascade of gene expression triggered by hypoxia, which in intact animals may act synergically with peripheral chemosensory inputs. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21837","type":"journal-article","created":{"date-parts":[[2008,8,27]],"date-time":"2008-08-27T20:21:38Z","timestamp":1219868498000},"page":"79-85","source":"Crossref","is-referenced-by-count":7,"title":["Glial vascular endothelial growth factor overexpression in rat brainstem under tolerable hypoxia: Evidence for a central chemosensitivity"],"prefix":"10.1002","volume":"87","author":[{"given":"J.S.","family":"Perrin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Araneda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Catteau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Autran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Denavit\u2010Saubi\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Pequignot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,8,27]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1995.75.1.1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406613200"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2004.02.010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2007.1713"},{"key":"e_1_2_5_7_1","first-page":"1029","article-title":"Vascular permeability factor\/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis","volume":"146","author":"Dvorak HF","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.08.011"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20420"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000102937.50486.1B"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00615-7"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00976"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/35093117"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10168"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20431"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016272"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2482-06.2007"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816x.2001.01816.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(04)81029-2"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00612185"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-4-00493.x"},{"key":"e_1_2_5_22_1","first-page":"245","article-title":"Effect of 5\u2010hydroxytryptamine on the peripheral chemoreceptors in the rat","volume":"16","author":"Sapru HN","year":"1977","journal-title":"Res Commun Chem Pathol Pharmacol"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.21837","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.21837","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T04:29:28Z","timestamp":1697084968000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.21837"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,27]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1002\/jnr.21837"],"URL":"https:\/\/doi.org\/10.1002\/jnr.21837","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,27]]}}}