{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:20:09Z","timestamp":1790281209404,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,10,24]],"date-time":"2008-10-24T00:00:00Z","timestamp":1224806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aquaporin\u20104 (Aqp4) is a water transport protein expressed in glia and ependymocytes in brain. We report here the unexpected occurrence of severe obstructive hydrocephalus in a random subset of <jats:italic>Aqp4<\/jats:italic> knockout mice. Of 612 <jats:italic>Aqp4<\/jats:italic> knockout mice produced by heterozygote\u2013heterozygote or knockout\u2013knockout breedings, 9.6% of offspring manifested progressive encephalomegaly. Encephalomegaly was never seen in wild\u2010type or <jats:italic>Aqp4<\/jats:italic> heterozygous mice. Examination of the subset encephalomegalic mice revealed marked triventricular hydrocephalus (lateral ventricle size \u223c500 mm<jats:sup>3<\/jats:sup>), elevated intracranial pressure (19 \u00b1 3 vs. 6.1 \u00b1 0.6 mm Hg), and death by age 6 weeks, with a median survival of 28 days. Intraventricular dye injection studies revealed total obstruction of the cerebral aqueduct. Evans blue extravasation studies indicated an intact blood\u2013brain barrier in the hydrocephalic mice. Brain histology revealed reduced ventricular size and ependymocyte disorganization in some nonhydrocephalic <jats:italic>Aqp4<\/jats:italic> null mice. Our studies establish <jats:italic>Aqp4<\/jats:italic> deletion as a predisposing factor for the development of congenital obstructive hydrocephalus in mice. We suggest that <jats:italic>AQP4<\/jats:italic> polymorphisms might also contribute to the development of aqueduct stenosis in humans. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.21927","type":"journal-article","created":{"date-parts":[[2008,10,24]],"date-time":"2008-10-24T20:55:14Z","timestamp":1224881714000},"page":"1150-1155","source":"Crossref","is-referenced-by-count":57,"title":["Sporadic obstructive hydrocephalus in <i>Aqp4<\/i> null mice"],"prefix":"10.1002","volume":"87","author":[{"given":"Xuechao","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marios C.","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongguo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"Verkman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonghui","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,10,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03362.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600306"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19980401)41:1<2::AID-JEMT2>3.0.CO;2-Z"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2004.tb00070.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20664"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI231"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/72256"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.04829.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0264.2007.00786.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-01-00171.1997"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00301016"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413627200"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-1723fje"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.20.11981"},{"issue":"6","key":"e_1_2_5_16_1","first-page":"459","article-title":"Expression of the water\u2010channel protein aquaporin 4 in the H\u2010Tx rat: possible compensatory role in spontaneously arrested hydrocephalus","volume":"105","author":"Shen XQ","year":"2006","journal-title":"J Neurosurg"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2007.11.003"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.02.018"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/62.10.1019"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e3282f2b4eb"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.21927","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.21927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T08:50:15Z","timestamp":1696927815000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.21927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,24]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1002\/jnr.21927"],"URL":"https:\/\/doi.org\/10.1002\/jnr.21927","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,24]]}}}