{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:45:53Z","timestamp":1773301553281,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,4,9]],"date-time":"2009-04-09T00:00:00Z","timestamp":1239235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2009,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neuronal apoptotic death involves the participation of reactive oxygen species (ROS), but their sources have not been completely elucidated. Previous studies have demonstrated that the ROS\u2010producing enzyme NADPH oxidase is present in neuronal cells and that this enzyme could participate in the apoptotic neuronal death. Cerebellar granule neurons (CGN) undergo apoptosis when cells are transferred from a medium with 25 mM KCl (K25) to a 5 mM KCl (K5) medium or when they are treated with staurosporine (ST). Under these conditions, apoptotic death of CGN is dependent on ROS production. In this study, we evaluated the role of NOX2, an NADPH oxidase homolog, in the apoptotic death of CGN induced by two different conditions. In CGN from NOX2\u2010deficient (ko) mice, a significantly lower rate of apoptotic death occurs compared with wild\u2010type (wt) CGN. Also, caspase\u20103 activation, NADPH oxidase activity, and superoxide anion production induced by ST were markedly lower in ko neurons than in wt CGN. In contrast to the case with ST, when CGN were treated with K5, no differences were observed between ko and wt cells in any of the parameters measured. However, all NADPH oxidase inhibitors tested noticeably reduced cell death and apoptotic parameters induced by K5 in both wt and ko CGN. These results suggest that NOX2 could be necessary for apoptotic death induced by ST, but not by K5, which could require other member of the NOX family in the apoptotic process. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.22079","type":"journal-article","created":{"date-parts":[[2009,4,9]],"date-time":"2009-04-09T21:15:46Z","timestamp":1239311746000},"page":"2531-2540","source":"Crossref","is-referenced-by-count":14,"title":["NOX2 mediates apoptotic death induced by staurosporine but not by potassium deprivation in cerebellar granule neurons"],"prefix":"10.1002","volume":"87","author":[{"given":"Alicia","family":"Guemez\u2010Gamboa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Mor\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19960215)43:4<465::AID-JNR7>3.0.CO;2-D"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00195-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2001.2642"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00044.2005"},{"key":"e_1_2_6_6_1","first-page":"736","volume-title":"Methods of enzymatic analysis","author":"Bergmeyer HU","year":"1968"},{"key":"e_1_2_6_7_1","first-page":"737","article-title":"Different sources of reactive oxygen species contribute to low potassium\u2010induced apoptosis in cerebellar granule cells","volume":"21","author":"Bobba A","year":"2008","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(03)00194-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10493"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1999.721394.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4306-04.2005"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.06.027"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.4.12.2233"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.20.13292"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200212200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-07-02203.1987"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R400024200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2001.0962"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1309"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20041835"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.01040.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200112131"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.04.021"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03189.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.07.023"},{"key":"e_1_2_6_27_1","first-page":"S28","article-title":"Tissue distribution and putative physiological function of NOX family NADPH oxidases","volume":"57","author":"Krause KH","year":"2004","journal-title":"Jpn J Infect Dis"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400387"},{"key":"e_1_2_6_29_1","first-page":"181","article-title":"Nox enzymes and the biology of reactive oxygen","volume":"4","author":"Lambeth JD","year":"2004","journal-title":"Nature"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.03.028"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606702200"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-006-9038-6"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.01023.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/20804"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.106.023937"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(89)90146-6"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1999.0730568.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68020750.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-23-j0001.2000"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2900041"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2003.03920.x"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.24.10703-10717.2004"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.06.020"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.70010316.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-15-04696.1996"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)03364-X"},{"key":"e_1_2_6_47_1","doi-asserted-by":"crossref","first-page":"9195","DOI":"10.1016\/S0021-9258(18)60513-7","article-title":"Activation of the superoxide forming NADPH oxidase in a cell\u2010free system by sodium dodecyl sulphate: absolute lipid dependence of the solubilized enzyme","volume":"264","author":"Shpungin S","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_6_48_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1523\/JNEUROSCI.20-01-j0006.2000","article-title":"NADPH contributes directly to oxidative stress and apoptosis in nerve growth factor\u2010deprived sympathetic neurons","volume":"20","author":"Tammariello S","year":"2000","journal-title":"J Neurosci"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.11.039"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2005.01.007"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)44045-X"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1312"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.1077"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.02.013"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.12.038"},{"key":"e_1_2_6_56_1","first-page":"2252","article-title":"Ischemic stroke injury is reduced in mice lacking a functional NADPH oxidase","volume":"28","author":"Walder CE","year":"1997","journal-title":"Am Heart Assoc"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.03.060"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.22079","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.22079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T08:33:30Z","timestamp":1697099610000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.22079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,9]]},"references-count":56,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,8,15]]}},"alternative-id":["10.1002\/jnr.22079"],"URL":"https:\/\/doi.org\/10.1002\/jnr.22079","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,9]]}}}