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In addition, axonal damage has also been observed in the brain of mice treated daily with DEDTC along postnatal development, though with this experimental model there was observed to be axonal recovery after treatment, during the adulthood. To focus on this axonal dynamic activity, damage\u2010recovery, a key axonal protein, the microtubule associated protein tau, was analyzed in this DEDTC model. Tau is a phosphoprotein and its dynamic site\u2010specific phosphorylation is essential for its proper function; in fact, high levels are correlated with cell dysfunction. Furthermore, the levels of tau phosphorylation are associated with dynamic microtubules during periods of high plasticity. Thus, phosphorylated tau at two sites of phosphorylation, Ser<jats:sup>199<\/jats:sup> and Ser<jats:sup>396<\/jats:sup>, were evaluated during the second week of postnatal development and throughout adulthood. The results obtained by Western blot made it evident that the levels of p\u2010tau Ser<jats:sup>199<\/jats:sup> and p\u2010tau Ser<jats:sup>396<\/jats:sup> were higher in treated mice than in controls. Interestingly, by immunohistochemistry there was shown to be an increase in p\u2010tau\u2010immunolabeling in neuronal soma together with axonal tract alterations in treated animals with respect to controls, and the analyses of GSK3\u03b2 and cdk5 revealed an increase in its activity in DEDTC\u2010treated animals. Nevertheless, in the adult a general decline in p\u2010tau was observed together with a rescue of axonal tract. All these data support the idea that the axonal damage induced by DEDTC treatment along postnatal development is followed by an axonal rescue during adulthood. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.22284","type":"journal-article","created":{"date-parts":[[2009,11,11]],"date-time":"2009-11-11T16:54:37Z","timestamp":1257958477000},"page":"1083-1093","source":"Crossref","is-referenced-by-count":2,"title":["Tau hyperphosphorylation and axonal damage induced by N,N\u2010diethyldithiocarbamate (DEDTC) treatment along late postnatal development is followed by a rescue during adulthood"],"prefix":"10.1002","volume":"88","author":[{"given":"Juana","family":"Utrera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Junyent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"de 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