{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T04:37:25Z","timestamp":1718080645063},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T00:00:00Z","timestamp":1263945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acoustic overstimulation causes apoptotic cell death in the cochlea. This death process is mediated, in part, by the mitochondrial signaling pathway involving Bcl\u20102 family proteins. Myeloid cell leukemia sequence 1 (Mcl\u2010l) is an antiapoptotic member of the Bcl\u20102 family. Its involvement in noise\u2010induced hair cell death has not been characterized. Here we report the endogenous expression and the noise\u2010induced expression of Mcl\u20101 in Sprague Dawley rat cochleae. In the sensory epithelia of normal cochleae, there is strong constitutive expression of Mcl\u20101 mRNA, with an expression level higher than that of many other Bcl\u20102 family genes. The Mcl\u20101 protein is preferentially expressed in outer hair cells. After exposure to a high level of continuous noise at 115\u2010dB sound pressure level for 1 hr, Mcl\u20101 expression displays a time\u2010dependent alteration, with up\u2010regulation of Mcl\u20101 mRNA at 4 hr postexposure and protein up\u2010regulation at 1 day postexposure. Western blot analysis reveals the up\u2010regulated Mcl\u20101 as the full\u2010length form of Mcl\u20101. Immunolabeling of the Mcl\u20101 protein reveals the early increase in Mcl\u20101 immunoreactivity in the nuclear region of the hair cells displaying apoptotic phenotypes and a subsequent increase in survival hair cells. These results suggest that Mcl\u20101 is involved in the regulation of hair cell pathogenesis resulting from acoustic stress, possibly by influencing the nuclear events of apoptosis. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.22333","type":"journal-article","created":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T15:47:14Z","timestamp":1264002434000},"page":"1812-1821","source":"Crossref","is-referenced-by-count":10,"title":["Acoustic overstimulation modifies Mcl\u20101 expression in cochlear sensory epithelial cells"],"prefix":"10.1002","volume":"88","author":[{"given":"Bo Hua","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunfeng","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M909826199"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2008.04.006"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.8.4883"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2004.04.018"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505688200"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2006.12.017"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402416"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0103020"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1156903"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20764"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.151.1.83"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2005.10.004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/000164800760370774"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(02)00286-1"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2008.08.006"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.03.072"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.7.3953"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(82)90017-X"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3516"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.68.1.158"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90098-1"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.7.2495"},{"key":"e_1_2_5_25_1","first-page":"99","volume-title":"Noise 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