{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T04:19:40Z","timestamp":1759033180222},"reference-count":69,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,4,5]],"date-time":"2010-04-05T00:00:00Z","timestamp":1270425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2010,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Injection of viral vectors into peripheral nerves may transfer specific genes into their dorsal root ganglion (DRG) neurons and motoneurons. However, myelin sheaths of peripheral axons block the entry of viral particles into nerves. We studied whether mild, transient peripheral nerve demyelination prior to intraneural viral vector injection would enhance gene transfer to target DRG neurons and motoneurons. The right sciatic nerve of C57BL\/6 mice was focally demyelinated with 1% lysolecithin, and the left sciatic nerve was similarly injected with saline (control). Five days after demyelination, 0.5 \u03bcl of Ad5\u2010GFP was injected into both sciatic nerves at the site of previous injection. The effectiveness of gene transfer was evaluated by counting GFP<jats:sup>+<\/jats:sup> neurons in the DRGs and ventral horns. After peripheral nerve demyelination, there was a fivefold increase in the number of infected DRG neurons and almost a 15\u2010fold increase in the number of infected motoneurons compared with the control, nondemyelinated side. Focal demyelination reduced the myelin sheath barrier, allowing greater virus\u2013axon contact. Increased CXADR expression on the demyelinated axons facilitated axoplasmic viral entry. No animals sustained any prolonged neurological deficits. Increased gene delivery into DRG neurons and motoneurons may provide effective treatment for amyotrophic lateral sclerosis, pain, and spinal cord injury. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.22394","type":"journal-article","created":{"date-parts":[[2010,4,5]],"date-time":"2010-04-05T16:33:19Z","timestamp":1270485199000},"page":"2374-2384","source":"Crossref","is-referenced-by-count":13,"title":["Enhanced adenoviral gene delivery to motor and dorsal root ganglion neurons following injection into demyelinated peripheral nerves"],"prefix":"10.1002","volume":"88","author":[{"given":"Yongjie","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiyan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa B.E.","family":"Shields","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panpan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darlene A.","family":"Burke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cai","family":"Jun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Byers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott R.","family":"Whittemore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher B.","family":"Shields","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20968"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02544"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20045"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5304.1320"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2006.23.508"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006123-199907000-00029"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3171\/spi.2002.96.2.0212"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2003.08.025"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1227\/01.NEU.0000044459.24519.3E"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.05.024"},{"key":"e_1_2_6_12_1","first-page":"326","article-title":"Replication\u2010defective genomic HSV gene therapy vectors: design, production and CNS applications","volume":"7","author":"Burton EA","year":"2005","journal-title":"Curr Opin Mol Ther"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960101)364:1<6::AID-CNE2>3.0.CO;2-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1104"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.707"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509062102"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/089771501750055811"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.sc.3101816"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2003.08.021"},{"key":"e_1_2_6_20_1","first-page":"132","article-title":"CNS gene delivery by retrograde transport of recombinant replication\u2010defective adenoviruses","volume":"2","author":"Ghadge GD","year":"1995","journal-title":"Gene Ther"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.835"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.1.442"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123709"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2005.02.005"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0497-429"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(98)00207-X"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(88)90026-7"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4908-07.2007"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(98)00042-2"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(78)90147-8"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2008.0543"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300367"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2000.00255.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2006.23.853"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.03.025"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.11.012"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10719"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08601.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03765.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/13550289509114022"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050950"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.4.1892-1903.2002"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(02)00131-4"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1993.1017"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)01065-3"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1153"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8382374"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199405000-00013"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00587-8"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302939"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.11.009"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2005.05.012"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2006.0004"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1789.2007.00767.x"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26210"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1089\/089771501300227314"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300774"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(02)00222-0"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.7.3.305"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.07.020"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302071"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00092.2006"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2006.178"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006123-200106000-00026"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/38410"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00776-005-0971-z"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1995.tb01054.x"},{"key":"e_1_2_6_68_1","first-page":"47","volume-title":"Dorsal root ganglion cells and their process","author":"Willis WD","year":"1991"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1089\/104303403765701187"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301185"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.22394","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.22394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T06:08:20Z","timestamp":1698905300000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.22394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,5]]},"references-count":69,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,8,15]]}},"alternative-id":["10.1002\/jnr.22394"],"URL":"https:\/\/doi.org\/10.1002\/jnr.22394","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,5]]}}}