{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T22:52:56Z","timestamp":1773096776374,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,2,2]],"date-time":"2011-02-02T00:00:00Z","timestamp":1296604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Binding of activated \u03b1<jats:sub>2<\/jats:sub>\u2010macroglobulin (\u03b1<jats:sub>2<\/jats:sub>M) to LDL receptor\u2010related protein\u20101 (LRP1) in Schwann cells activates ERK\/MAP kinase and Akt and thereby promotes cell survival and migration. The goal of this study was to determine whether \u03b1<jats:sub>2<\/jats:sub>M binding to LRP1 regulates expression of cytokines and chemokines. To assess the LRP1 response selectively, we studied primary cultures of rat Schwann cells. In a screening assay that detects 84 gene products, monocyte chemoattractant protein\u20101 (MCP\u20101\/CCL2) mRNA expression was increased more than 13\u2010fold in Schwann cells treated with activated \u03b1<jats:sub>2<\/jats:sub>M. The effects of \u03b1<jats:sub>2<\/jats:sub>M on MCP\u20101 expression were selective, because expression of the general proinflammatory cytokine tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) was not induced. We confirmed that \u03b1<jats:sub>2<\/jats:sub>M selectively induces expression of MCP\u20101 and not TNF\u2010\u03b1 in single\u2010target qPCR assays. MCP\u20101 protein accumulated at increased levels in conditioned medium of \u03b1<jats:sub>2<\/jats:sub>M\u2010treated cells. LRP1 was necessary for induction of MCP\u20101 expression, as determined in experiments with the LRP1 antagonist receptor\u2010associated protein, a mutated form of full\u2010length \u03b1<jats:sub>2<\/jats:sub>M that does not bind LRP1, and in studies with Schwann cells in which LRP1 was silenced. Inhibiting ERK\/MAP kinase activation blocked expression of MCP\u20101. These studies support a model in which LRP1 regulates multiple aspects of Schwann cell physiology in the response to PNS injury. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.22576","type":"journal-article","created":{"date-parts":[[2011,2,2]],"date-time":"2011-02-02T18:47:09Z","timestamp":1296672429000},"page":"544-551","source":"Crossref","is-referenced-by-count":19,"title":["Regulation of cytokine expression by Schwann cells in response to \u03b1<sub>2<\/sub>\u2010macroglobulin binding to LRP1"],"prefix":"10.1002","volume":"89","author":[{"given":"Yang","family":"Shi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomonori","family":"Yamauchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alban","family":"Gaultier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinako","family":"Takimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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