{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T11:58:39Z","timestamp":1773403119336,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T00:00:00Z","timestamp":1329868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>NMO\u2010IgG autoantibody selectively binds to aquaporin\u20104 (AQP4), the most abundant water channel in the central nervous system and is now considered a useful serum biomarker of neuromyelitis optica (NMO). A series of clinical and pathological observations suggests that NMO\u2010IgG may play a central role in NMO physiopathology. The current study evaluated, in well\u2010differentiated astrocytes cultures, the consequences of NMO\u2010IgG binding on the expression pattern of AQP4 and on plasma membrane water permeability. To avoid or to facilitate AQP4 down\u2010regulation, cells were exposed to inactivated sera in two different situations (1 hr at 4\u00b0C or 12 hr at 37\u00b0C). AQP4 expression was detected by immunofluorescence studies using a polyclonal anti\u2010AQP4 or a human anti\u2010IgG antibody, and the water permeability coefficient was evaluated by a videomicroscopy technique. Our results showed that, at low temperatures, cell exposure to either control or NMO\u2010IgG sera does not affect either AQP4 expression or plasma membrane water permeability, indicating that the simple binding of NMO\u2010IgG does not affect the water channel's activity. However, at 37\u00b0C, long\u2010term exposure to NMO\u2010IgG induced a loss of human IgG signal from the plasma membrane along with M1\u2010AQP4 isoform removal and a significant reduction of water permeability. These results suggest that binding of NMO\u2010IgG to cell membranes expressing AQP4 is a specific mechanism that may account for at least part of the pathogenic process. \u00a9 2012 Wiley Priodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.22822","type":"journal-article","created":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T05:37:35Z","timestamp":1329889055000},"page":"1240-1248","source":"Crossref","is-referenced-by-count":27,"title":["Neuromyelitis Optica Immunoglobulin G present in sera from neuromyelitis optica patients affects aquaporin\u20104 expression and water permeability of the astrocyte plasma membrane"],"prefix":"10.1002","volume":"90","author":[{"given":"Luciana","family":"Melamud","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan 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