{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:09:40Z","timestamp":1790248180963,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T00:00:00Z","timestamp":1334793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endothelial progenitor cells (EPC) have been associated with good functional outcome in ischemic stroke. From preclinical studies, it has been reported that EPC proliferation is mediated by several molecular markers, including vascular endothelial growth factor (VEGF), stromal cell\u2010derived factor\u20101\u03b1 (SDF\u20101\u03b1), and the activity of matrix metalloproteinase\u20109 (MMP\u20109). Therefore, our aim was to study the role of these molecular factors in EPC proliferation in human ischemic stroke. Forty\u2010eight patients with first episode of nonlacunar ischemic stroke were prospectively included in the study within 12 hr of symptom onset. EPC colonies were classified as early\u2010outgrowth colony forming unit\u2010endothelial cell (CFU\u2010EC) and quantified at admission, at 24 and 72 hr, at day 7, and at 3 months. At the same time, serum levels of VEGF, SDF\u20101\u03b1, and active MMP\u20109 were measured by ELISA. The primary endpoint was EPC increment during the first week, which was defined as the difference in the number of CFU\u2010EC between day 7 and admission. We found that VEGF (r = 0.782), SDF\u20101\u03b1 (r = 0.828), and active MMP\u20109 (r = 0.740) levels at 24 hr from stroke onset showed a strong correlation with EPC increment. Similar results were found for VEGF levels at 72 hr (r = 0.839) and at day 7 (r = 0.602) as well as for active MMP\u20109 levels at 72 hr (r = 0.442) and at day 7 (r = 0.474). In the multivariate analyses, serum levels of VEGF at 72 hr (B: 0.074, <jats:italic>P<\/jats:italic> &lt; 0.0001) and SDF\u20101\u03b1 at 24 hr (B: 0.049, <jats:italic>P<\/jats:italic> = 0.008) were independent factors for EPC increment during the first week of evolution. These findings suggest that VEGF and SDF\u20101\u03b1 may mediate EPC proliferation in human ischemic stroke. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23068","type":"journal-article","created":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T11:00:10Z","timestamp":1334833210000},"page":"1788-1793","source":"Crossref","is-referenced-by-count":43,"title":["Temporal profile of molecular signatures associated with circulating endothelial progenitor cells in human ischemic 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