{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T17:14:12Z","timestamp":1751476452896},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,14]],"date-time":"2012-11-14T00:00:00Z","timestamp":1352851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Protein kinase C\u03b1 (PKC\u03b1) has been implicated in the regulation of a variety of cellular functions, such as proliferation, differentiation, and apoptosis, in response to a diverse range of stimuli. Activated PKC\u03b1 mediates oxidative stress, apoptosis, and inflammatory reaction. Thyroid hormone (TH) is essential for the proper development of the mammalian central nervous system. TH deficiency during critical periods of brain development results in permanent cognitive and neurological impairments. In the present study, we attempted to explore whether PKC\u03b1 is involved in impaired brain function in developing hypothyroid rat brain. Severe perinatal hypothyroidism was obtained by administration of 30 mg\/day propylthiouracil to dams. Brain PKC activity in hypothyroid pups was increased significantly in cytosol and membrane fractions. The change of membrane PKC activity was more marked than that of cytosol, and hypothyroidism led to a higher ratio of membrane PKC activity to that in cytosol, which means abnormal activation of PKC in developing hypothyroid rat brain. Thyroxine replacement partially corrected these changes. After being treated with bisindolmaleimide XI, a mainly selective inhibitor for PKC\u03b1, the hypothyroid pups showed improved place navigation test results, and further Western blot analysis showed that PKC\u03b1 expression in cytosol fractions was increased in hypothyroid rat brain with or without bisindolmaleimide XI treatment, but, after treatment with bisindolmaleimide XI, PKC\u03b1 content in membrane fractions decreased almost to normal. Therefore, we conclude that PKC\u03b1 appears to be involved in the impaired brain development observed in perinatal hypothyroid rat brain. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23125","type":"journal-article","created":{"date-parts":[[2012,11,14]],"date-time":"2012-11-14T15:46:33Z","timestamp":1352907993000},"page":"211-219","source":"Crossref","is-referenced-by-count":2,"title":["Protein kinase c\u03b1 is involved in impaired perinatal hypothyroid rat brain development"],"prefix":"10.1002","volume":"91","author":[{"given":"Hong\u2010Mei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2004.08.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/105072503770867174"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2010.01.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1523\/JNEUROSCI.08-02-00701.1988","article-title":"Immunocytochemical localization of the guanine nucleotide\u2010binding protein Go in primary cultures of neuronal and glial cells","volume":"8","author":"Brabet P","year":"1988","journal-title":"J Neurosci"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02116-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02495.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90368-W"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00221-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.1.13"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-2281-y"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03345560"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000077407.90309.65"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3181\/0705-MR-134"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-2-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1164"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2009.10.006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0027"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ntt.2011.04.001"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"4766","DOI":"10.1016\/S0021-9258(18)83655-9","article-title":"Stimulation in vitro of rabbit erythrocyte cytosol phospholipid\u2010dependent protein kinase activity. 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