{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:40:36Z","timestamp":1758631236385},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T00:00:00Z","timestamp":1367280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p>Dopaminergic (DA) neuron therapy has been established as a new clinical tool for treating Parkinson's disease (PD). Prior to cell transplantation, there are two primary issues that must be resolved: one is the appropriate seed cell origin, and the other is the efficient inducing technique. In the present study, human umbilical cord blood\u2010derived mesenchymal stem cells (hUCB\u2010MSCs) were used as the available seed cells, and conditioned medium from human amniotic epithelial cells (ACM) was used as the inducing reagent. Results showed that the proportion of DA neuron\u2010like cells from hUCB\u2010MSCs was significantly increased after cultured in ACM, suggested by the upregulation of DAT, TH, Nurr1, and Pitx3. To identify the process by which ACM induces DA neuron differentiation, we pretreated hUCB\u2010MSCs with k252a, the Trk receptor inhibitor of brain\u2010derived neurotrophic factor (BDNF) and nerve growth factor (NGF), and found that the proportion of DA neuron\u2010like cells was significantly decreased compared with ACM\u2010treated hUCB\u2010MSCs, suggesting that NGF and BDNF in ACM were involved in the differentiation process. However, we could not rule out the involvement of other unidentified factors in the ACM, because ACM\u2009+\u2009k252a treatment does not fully block DA neuron\u2010like cell differentiation compared with control. The transplantation of ACM\u2010induced hUCB\u2010MSCs could ameliorate behavioral deficits in PD rats, which may be associated with the survival of engrafted DA neuron\u2010like cells. In conclusion, we propose that hUCB\u2010MSCs are a good source of DA neuron\u2010like cells and that ACM is a potential inducer to obtain DA neuron\u2010like cells from hUCB\u2010MSCs in vitro for an ethical and legal cell therapy for PD. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23225","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T13:36:10Z","timestamp":1367328970000},"page":"978-986","source":"Crossref","is-referenced-by-count":24,"title":["Conditioned medium from human amniotic epithelial cells may induce the differentiation of human umbilical cord blood mesenchymal stem cells into dopaminergic neuron\u2010like cells"],"prefix":"10.1002","volume":"91","author":[{"given":"Shu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Mei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Shuai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Qing","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De\u2010Shan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology School of Basic Medical Sciences, Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.01.032"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2007.0172"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)64033-9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2005.12.042"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240701196829"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)03217-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.01986.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.06.111"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/bbmt.2001.v7.pm11760145"},{"key":"e_1_2_6_11_1","first-page":"36","article-title":"Differentiation of mesenchymal stem cells into dopaminergic neuron\u2010like cells in vitro","volume":"18","author":"Guo L","year":"2005","journal-title":"Biomed Environ Sci"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010209107"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/350230a0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-004-0918-z"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040440"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1670"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21669"},{"key":"e_1_2_6_18_1","first-page":"1099","article-title":"Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood","volume":"86","author":"Mareschi K","year":"2001","journal-title":"Haematologica"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.126286"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0230529100"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051285"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.85.20"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/527"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2004.11.007"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2039"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-211-45295-0_10"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.12.090"},{"key":"e_1_2_6_28_1","first-page":"371","article-title":"K252a is a selective inhibitor of the tyrosine protein kinase activity of the trk family of oncogenes and neurotrophin receptors","volume":"7","author":"Tapley P","year":"1992","journal-title":"Oncogene"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009398"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20001115)62:4<585::AID-JNR13>3.0.CO;2-U"},{"key":"e_1_2_6_31_1","first-page":"269","article-title":"Stimulation of nerve growth factor expression in astrocytes by peroxynitrite","volume":"18","author":"Vargas MR","year":"2004","journal-title":"In Vivo"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006606"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0258"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.04.046"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031999"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21649"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.23225","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.23225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T11:52:25Z","timestamp":1700653945000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.23225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,30]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/jnr.23225"],"URL":"https:\/\/doi.org\/10.1002\/jnr.23225","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,30]]}}}