{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:43:44Z","timestamp":1789587824167,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T00:00:00Z","timestamp":1384819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fundaci\u00f3n Alberto J. Roemmers, University of Buenos Aires","award":["UBACYT 200-20110100034"],"award-info":[{"award-number":["UBACYT 200-20110100034"]}]},{"name":"Fundaci\u00f3n Alberto J. Roemmers, University of Buenos Aires","award":["UBACYT: 200-20100100329"],"award-info":[{"award-number":["UBACYT: 200-20100100329"]}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"crossref","award":["PIP 11220110101089"],"award-info":[{"award-number":["PIP 11220110101089"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p>Although HIV\u2010associated neurocognitive disorders (HAND) result from injury and loss of neurons, productive infection routinely takes place in cells of macrophage lineage. In such a complex context, astrocytosis induced by local chemokines\/cytokines is one of the hallmarks of HIV neuropathology. Whether this sustained astrocyte activation is able to alter telomere\u2010aging process is unknown. We hypothesized that interaction of HIV with astrocytes may impact astrocyte telomerase activity (TA) and telomere length in a scenario of astrocytic activation measured by expression of glial fibrillary acidic protein (GFAP). To test this hypothesis, cultured murine astrocytes were challenged with pseudotyped HIV\/vesicular stomatitis virus (HIV\/VSV) to circumvent the absence of viral receptors; and GFAP, telomerase activity, and telomere length were quantified. As an early and transient event after HIV infection, both TA activity and telomere length were significantly augmented (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Later, a strong negative correlation (\u22120.8616, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001) between virus production and telomerase activity was demonstrated. Once HIV production had reached a peak (7 dpi), the TA decreased, showing levels similar to those of noninfected cells. In contrast, the astrocyte became activated, exhibiting significantly increased levels of GFAP expression directly related to the level of HIV\/VSV replication (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). Our results suggest that HIV\u2010infected astrocytes exhibit early disturbance in their cellular functions, such as telomerase activity and telomere length, that may attenuate cell proliferation and enhance the astrocyte dysregulation, contributing to HIV neuropathogenesis. Understanding the mechanisms involved in HIV\u2010mediated persistence by altering the telomere\u2010related aging processes could aid in the development of therapeutic modalities for neurological complications of HIV infection. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23294","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T15:33:00Z","timestamp":1384875180000},"page":"267-274","source":"Crossref","is-referenced-by-count":18,"title":["Increased in vitro glial fibrillary acidic protein expression, telomerase activity, and telomere length after productive human immunodeficiency virus\u20101 infection in murine astrocytes"],"prefix":"10.1002","volume":"92","author":[{"given":"Diego","family":"Ojeda","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas en Retrovirus y Sida, Universidad de Buenos Aires\u2010CONICET Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Jos\u00e9","family":"L\u00f3pez\u2010Costa","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Celular y Neurociencias \u201cProf. E. 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