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Dysfunction of either system results in developmental abnormalities and even death in animals. This study investigates whether and how proteasome inhibition impacts the components of the calreticulin (CRT)\/calnexin (CNX) glycoprotein folding machinery, a typical ER protein quality\u2010control system, in the context of early neuronal injury. Here we report that proteasome inhibitor treatments, at nonlethal levels, reduced protein levels of CRT and ERp57 but not of CNX. These treatments increased protein levels of CRT in culture media, an effect blocked by brefeldin A, an inhibitor of protein trafficking; by contrast, ERp57 was not detected in culture media. Knockdown of CRT levels alone increased the vulnerability of SH\u2010SY5Y, a neuronal cell line, to 6\u2010hydroxydopamine (6\u2010OHDA) toxicity. In a rat model of Parkinson's disease, intrastriatal 6\u2010OHDA lesions resulted in decreased levels of CRT and ERp57 in the midbrain. These findings suggest that reduction of the components of CRT\/CNX glycoprotein quality\u2010control system may play a role in neuronal injury in Parkinson's disease and other neurodegenerative disorders associated with dysfunction of the ubiquitin\u2010proteasome system. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23413","type":"journal-article","created":{"date-parts":[[2014,5,23]],"date-time":"2014-05-23T11:52:50Z","timestamp":1400845970000},"page":"1319-1329","source":"Crossref","is-referenced-by-count":21,"title":["Reductions of the components of the calreticulin\/calnexin quality\u2010control system by proteasome inhibitors and their relevance in a rodent model of Parkinson's disease"],"prefix":"10.1002","volume":"92","author":[{"given":"Xiu\u2010Li","family":"Kuang","sequence":"first","affiliation":[{"name":"School of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang People's Republic of China"},{"name":"State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang People's Republic of China"},{"name":"State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang People's Republic of China"},{"name":"State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimei","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang People's Republic of China"},{"name":"State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang People's Republic of China"},{"name":"State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Shan","sequence":"additional","affiliation":[{"name":"School of Life Sciences and CAS Key Laboratory of Brain Function and Disease University of Science and Technology of China Hefei Anhui Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology Washington University School of Medicine St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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