{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T13:13:48Z","timestamp":1772889228271,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T00:00:00Z","timestamp":1405555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30901924"],"award-info":[{"award-number":["30901924"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science foundation of Zhejiang Province","award":["Y12H270010"],"award-info":[{"award-number":["Y12H270010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>P2X is a family of ligand\u2010gated ion channels that act through adenosine ATP. The P2X<jats:sub>3<\/jats:sub> receptor plays a key role in the transmission of neuropathic pain at peripheral and spinal sites. Electroacupuncture (EA) has been used to treat neuropathic pain effectively. To determine the role of EA in neuropathic pain mediated through the P2X<jats:sub>3<\/jats:sub> receptor in dorsal root ganglion neurons and the spinal cord, a chronic constriction injury (CCI) model was used. Sprague\u2010Dawley rats were divided into four groups: sham CCI, CCI, CCI plus contralateral EA, and CCI plus ipsilateral EA. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were recorded. Furthermore, the expression of the P2X<jats:sub>3<\/jats:sub> receptor was evaluated through Western blotting and immunofluorescence. The effects of EA and A\u2010317491 were investigated through the whole\u2010cell patch\u2010clamp method and intrathecal administration. Our results show that the MWT and TWL of EA groups were higher than those in the CCI group, whereas the expression of the P2X<jats:sub>3<\/jats:sub> receptor was lower than that in the CCI group. However, no significant difference was detected between the two EA groups. EA depressed the currents created by ATP and the upregulation of the P2X<jats:sub>3<\/jats:sub> receptor in CCI rats. Additionally, EA was more potent in reducing mechanical allodynia and thermal hyperalgesia when combined with A\u2010317491 through intrathecal administration. These results show that both contralateral and ipsilateral EA might inhibit the primary afferent transmission of neuropathic pain induced through the P2X<jats:sub>3<\/jats:sub> receptor. In addition, EA and A\u2010317491 might have an additive effect in inhibiting the transmission of pain mediated by the P2X<jats:sub>3<\/jats:sub> receptor. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23451","type":"journal-article","created":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T04:59:15Z","timestamp":1405573155000},"page":"1703-1713","source":"Crossref","is-referenced-by-count":51,"title":["Electroacupuncture and A\u2010317491 depress the transmission of pain on primary afferent mediated by the P2X<sub>3<\/sub> receptor in rats with chronic neuropathic pain states"],"prefix":"10.1002","volume":"92","author":[{"given":"Wan\u2010Sheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou 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