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The catalytic GII\u03b1 subunit, with the help of mannose 6\u2010phosphate receptor homology domain of GII\u03b2, sequentially hydrolyzes two \u03b11\u20103\u2010linked glucose residues in the second step of N\u2010linked oligosaccharide\u2010mediated protein folding. The soluble GII\u03b1 subunit is retained in the ER through its interaction with the HDEL\u2010containing GII\u03b2 subunit. N\u2010glycosylation and correct protein folding are crucial for protein stability and trafficking and cell surface expression of several proteins in the brain. Alterations in N\u2010glycosylation lead to abnormalities in neuronal migration and mental retardation, various neurodegenerative diseases, and invasion of malignant gliomas. Inhibitors of GII are used to inhibit cell proliferation and migration in a variety of different pathologies, such as viral infection, cancer, and diabetes. Despite the widespread use of GII\u03b1 inhibitory drugs and the role of GII\u03b1 in brain function, little is known about its expression in brain and other tissues. Here, we report generation of a highly specific chicken antibody to the GII\u03b1 subunit and its characterization by Western blotting and immunoprecipitation using cerebral cortical extracts. By using this antibody, we showed that the GII\u03b1 protein is highly expressed in testis, kidney, and lung, with the lowest amount in heart. GII\u03b1 polypeptide levels in whole brain were comparable to those in spleen. However, a higher expression of GII\u03b1 protein was detected in the cerebral cortex, reflecting its continuous requirement in correct folding of cell surface proteins. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23470","type":"journal-article","created":{"date-parts":[[2014,8,18]],"date-time":"2014-08-18T06:04:09Z","timestamp":1408341849000},"page":"82-93","source":"Crossref","is-referenced-by-count":3,"title":["Expression of \u03b1\u2010subunit of \u03b1\u2010glucosidase II in adult mouse brain regions and selected organs"],"prefix":"10.1002","volume":"93","author":[{"given":"Antje","family":"Anji","sequence":"first","affiliation":[{"name":"Department of Anatomy and Physiology CVM, Kansas State University Manhattan Kansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayley","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology CVM, Kansas State University Manhattan 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