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At 6 months of age, male mice were given either sham or drug i.p. injections for 3 days at a dose of 2 mg\/kg each day. On the day after the final injection, mice were subjected to behavioral testing before brain collection. Left hemisphere hippocampi were collected from all mice for protein analysis via Western blot. Right brain hemispheres were fixed and embedded in gelatin and then serially sectioned. The sections were immunostained with anti\u2010c\u2010Fos antibodies. Prostaglandin analysis was performed from remaining homogenized brain samples, minus the hippocampi. Injection of SQ 29,548 decreased selective brain prostaglandin levels compared with sham controls. This correlated with robust increases in limbic\u2010region c\u2010Fos immunoreactivity in the SQ 29,548\u2010injected mice. However, drug\u2010treated mice demonstrated no significant changes in relevant hippocampal protein levels compared with sham treatments, as determined from Western blots. Surprisingly, injection of SQ 29,548 caused mixed changes in parameters of depression and anxiety\u2010like behavior in the mice. In conclusion, the results indicate that administration of peripheral TP receptor antagonists alters brain levels of prostanoids and influences neuronal activity, with only minimal alterations of behavior. Whether the drug affects neurons directly or through a secondary pathway involving endothelium or other tissues remains unclear. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23578","type":"journal-article","created":{"date-parts":[[2015,2,23]],"date-time":"2015-02-23T04:51:02Z","timestamp":1424667062000},"page":"1279-1292","source":"Crossref","is-referenced-by-count":3,"title":["Brain changes associated with thromboxane receptor antagonist SQ 29,548 treatment in a mouse model"],"prefix":"10.1002","volume":"93","author":[{"given":"Andrew A.","family":"Rebel","sequence":"first","affiliation":[{"name":"Department of Biology University of North Dakota School of Medicine and Health Sciences Grand Forks North Dakota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siri A.","family":"Urquhart","sequence":"additional","affiliation":[{"name":"Department of Biology University of North Dakota School of Medicine and Health Sciences Grand Forks North 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